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- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 免疫类型：
IgG2b
- 细胞形态：
淋巴样
- 生长状态：
悬浮生长
- 库存：
大量
- ATCC Number：
TIB-210™
- 物种来源：
大鼠
- 是否是肿瘤细胞：
0
| Designations: | 2.43 | ||
| Depositors: | FW Fitch | ||
| Isotype: | IgG2b | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against Lyt-2.2 (mouse cytotoxic, suppressor T cell antigen) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with CTL Clone L3 cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 x 10 exp4 cells/ml and maintain between 4 x 10 exp4 and 5 x 10 exp5 cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 984: Sarmiento M, et al. IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing lyt 2 antigen block T-cell mediated cytolysis in the absence of complement. J. Immunol. 125: 2665-2672, 1980. PubMed: 6159417 33098: Sayles PC, Johnson LL. Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838 58011: Sarmiento M, et al. Cloned T lymphocytes and monoclonal antibodies as probes for cell surface molecules active in T cell-mediated cytolysis. Immunol. Rev. 68: 135-169, 1982. PubMed: 6184304 |
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