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- 细胞形态：
成纤维样
- 年限：
1 week
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
成纤维细胞
- 相关疾病：
正常
- ATCC Number：
CRL-1509™
- 生长状态：
贴壁生长
- 库存：
大量
- 器官来源：
皮肤
| Designations: | CCD-43Sk | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: skin Disease: normal Cell Type: fibroblast |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | 1 week | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The line was derived from normal skin of a child that died of necrotizing enterocolitis. The line has undergone approximately 21 population doublings in culture. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days. |
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文献和实验(destaining/exocytosis ). Using a fluorescence microscope attached to a CCD camera or a confocal, it is possible to follow secretion in live cell or tissue preparations and in this chapter, we will make a description of FM1-43 staining and destaining protocol
sapiens (human) LS 180 CL-188 Homo sapiens (human) LS 174T CRL-10624 Homo sapiens (human) CO 88BV59-1 CRL-11538 Homo sapiens (human) Co88BV59H21-2 CRL-11539 Homo sapiens (human) Co88BV59H21-2V67-66 CRL-1459 Homo sapiens (human) CCD-18
日本工程师ENGINEER DZ-25 日本工程师ENGINEER 日本工程师ENGINEER KA-15 日本工程师ENGINEER KA-16 日本工程师ENGINEER KA-17 日本工程师ENGINEER KA-22 日本工程师ENGINEER KA-30 日本工程师ENGINEER KA-42 日本工程师ENGINEER KA-43 日本工程师ENGINEER KA-44 日本工程师ENGINEER
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