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- 技术资料
- 免疫类型：
kappa light chain
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
- 生长状态：
悬浮生长
- 物种来源：
大鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
B淋巴细胞
- 库存：
大量
- 相关疾病：
多发性骨髓瘤
- ATCC Number：
CRL-1631™
- 品系：
Lou
| Designations: | Y3-Ag 1.2.3 | ||
| Depositors: | K Yamada | ||
| Isotype: | kappa light chain | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Rattus norvegicus deposited as Rattus sp. | ||
| Morphology: | lymphoblast |
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| Source: | Disease: plasmacytoma; myeloma Strain: Lou Cell Type: B lymphoblast; |
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| Cellular Products: | immunoglobulin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | the depositor has provided these cells for research purposes only and with the following restrictions: (1) no distribution should be made to third parties, (2) any proposed commercial use must be negotiated with the National Research Development Corporation, 101 Newington Causeway, London SE1 6BU, (3) all papers reporting any use of these or derived clones should make direct reference to the original publication (Nature 277:131-133, 1979) | ||
| Comments: | This is a derivative of the S210 myeloma developed by C. Milstein. No commercial use or third party distribution is permitted without the concurrence of C. Milstein. The cells are resistant to 8-azaguanine, are HAT sensitive and can be used as a fusion partner for rat B cells to make rat - rat hybridomas. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 1090: Galfre G, et al. Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG. Nature 277: 131-133, 1979. PubMed: 310519 26115: Bazin H, et al. Transplantable immunoglobulin-secreting tumours in rats. I. General features of LOU-Ws1 strain rat immunocytomas and their monoclonal proteins. Int. J. Cancer 10: 568-580, 1972. PubMed: 4668496 |
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文献和实验前体RNA中参与内含子剪接的两个特殊位点，即在内含子和外显子交界处有两个相当短的保守序列:5＇端为GT, 3＇端为 AG,称为GT-AG规律。GT-AG规则主要适用于(或是全部)真核生物基因的剪接位点。说明内含子的切除有一共同的机理。应指出的是，这种保守性不适用于线粒体、叶绿体和酵母tRNA基因转录后的加工。
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