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- 相关疾病：
其他疾病
- ATCC Number：
CRL-2053™
- 库存：
大量
- 年限：
10 days
- 器官来源：
睾丸
- 运输方式：
冻存运输
- 细胞形态：
上皮样
- 物种来源：
转基因小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 生长状态：
贴壁生长
- 品系：
BALB/c
| Designations: | GC-1 spg | ||
| Depositors: | MC Hofmann | ||
| Biosafety Level: | 2 [Cells contain SV-40 viral DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus , transgenic for SV40 early region deposited as mouse, transgenic for SV40 early region | ||
| Morphology: | epithelial |
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| Source: | Organ: testis Strain: BALB/c Disease: spermatogonia Cell Type: transformed with pSV3-neo |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | 10 days | ||
| Gender: | male | ||
| Comments: | Type B spermatogonia were immortalized by transfection with pSV3-neo (a plasmid containing coding sequences for the SV40 large T antigen and neomycin resistance). The line shows characteristics of a stage between type B spermatogonia and primary spermatocytes. The cells express two testis specific isoproteins, cytochrome c and lactate dehydrogenase C4. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
|
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 23124: Hofmann MC, et al. Immortalization of germ cells and somatic testicular cells using the SV40 large T antigen. Exp. Cell Res. 201: 417-435, 1992. PubMed: 1322317 | ||
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文献和实验Use of Lectins for Characterization of O-Linked Glycans of Herpes Simplex Virus Glycoproteins
of HSV-1 (designated gC-1) was demonstrated to contain domains, in which numerous O -linked glycans were concentrated to pronase-resistant clusters (5 -7 ), thereby resembling the organization of mucins (8 ). Thus glycoprotein, containing nine sites
Herpes Simplex Virus-Cell Interactions Studied by Immunogold Cryosection Electron Microscopy
; (3 ) the membraneous ultrastructure and immunolabeling are well preserved; (4 ) immunolabeling is less in the two-layer method; (5 ) silver-enhanced gold particles vary in size and shape; (6 ) it is possible to demonstrate herpes simplex virus type 1 glycoproteins gC-1
5. 5) 下,质粒结合至亲和载体颗粒上,溶液中高浓度的NaCl 可稳定三联体形式并减少与蛋白质、细胞DNA 的非特异性结合。经一段时间反应后,颗粒悬浮液被加至一层析柱 ,用适当的洗脱液改变p H 值至碱性环境,可使三联体解聚,质粒被洗脱。经该法分离质粒DNA ,质粒产量可达到加入量的62 %。 也有用Schizophyllan ( SPG) 制备亲和层析柱分离纯化 RNA 的报道。SPG是一种β21 ,32葡聚糖,在低温下,含RNA 的流动相通过层析柱,Poly (C) 和poly
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