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- 相关疾病：
其他疾病
- 器官来源：
肺
- 库存：
大量
- 运输方式：
冻存运输
- ATCC Number：
CRL-1642™
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞形态：
其他
- 品系：
C57BL
- 生长状态：
混合型生长
| Designations: | LL/2 (LLC1) | ||
| Depositors: | JS Bertram | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | mixed, adherent and suspension | ||
| Organism: | Mus musculus | ||
| Morphology: | rounded - loosely attached or floating |
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| Source: | Strain: C57BL Organ: lung Disease: Lewis lung carcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma. Tested and found negative for ectromelia virus (mousepox). The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate. This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents. |
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| Tumorigenic: | Yes | ||
| Antigen Expression: | H-2b | ||
| Comments: | This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents. Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma. The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate. The cells are reported to be highly tumorigenic, but weakly metastatic in mice. The cells form multilayers in flasks without actually becoming confluent. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Subcultures are prepared by diluting the suspension 1:4 to 1:6. Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with 0.25% trypsin - 0.53 mM EDTA solution. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 21 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 1091: Bertram JS, Janik P. Establishment of a cloned line of Lewis lung carcinoma cells adapted to cell culture. Cancer Lett. 11: 63-73, 1980. PubMed: 7226139 45212: Sharma S, et al. T cell-derived IL-10 promotes lung cancer growth by suppressing both T cell and APC function. J. Immunol. 163: 5020-5028, 1999. PubMed: 10528207 |
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