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- 详细信息
- 询价记录
- 技术资料
- 运输方式：
冻存运输
- 物种来源：
转基因小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- ATCC Number：
CRL-2618™
- 年限：
6 months
- 生长状态：
贴壁生长
- 库存：
大量
- 器官来源：
睾丸
| Designations: | 15P-1 | ||
| Depositors: | F Cuzin | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus , transgenic deposited as mouse, transgenic | ||
| Morphology: | |
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| Source: | Organ: testis Cell Type: Sertoli cell; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | 6 months | ||
| Gender: | male | ||
| Comments: | The 15P-1 cell line was established from testicular cells of transgenic mice that express the large T protein of polyoma virus (PyLT) in the seminiferous epithelium. [53202 ] This line exhibits characteristics of Sertoli cells, including transcription of the Wilms' tumor (WT1) and Steel genes. [53203 ] 15P-1 cultures express phagocytic activity evidenced by the internalization of latex beads and the disposal of dead cells. Interaction with germ cells enhances the phagocytic activity of 15P-1 cells. [53204 ] The cells support the meiotic and postmeiotic differentiation in cocultures of diploid premeiotic germ cells into haploid spermatids expressing the protamine (Prm-1) gene. [53203 ] When cocultured with 15P-1 cells, testicular cells explanted from immature 9-day-old animals, before the onset of the first meiosis, generate tetrads of haploid cells with the morphology of round spermatids and initiate protamine transcription. [53203 ] The 15P-1 cell line releases protease-sensitive material with broad-spectrum antibacterial activity. The activity of 15P-1 culture medium is increased 10-fold to 50-fold in the presence of fractions enriched in round spermatids and of nerve growth factor. [53205 ] The KL protein, a ligand of the Kit receptor, is expressed in Sertoli cells and in 15P-1 cells. [53206 ] |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 32.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Do not inoculate new flasks at cell densities below 1 X 10 exp4 cells per sq. cm. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 53202: Paquis-Flucklinger V, et al. Expression in transgenic mice of the large T antigen of polyomavirus induces Sertoli cell tumours and allows the establishment of differentiated cell lines. Oncogene 8: 2087-2094, 1993. PubMed: 8393161 53203: Rassoulzadegan M, et al. Transmeiotic differentiation of male germ cells in culture. Cell 75: 997-1006, 1993. PubMed: 7504588 53204: Grandjean V, et al. Stage-specific signals in germ line differentiation: control of Sertoli cell phagocytic activity by spermatogenic cells. Dev. Biol. 184: 165-174, 1997. PubMed: 9142992 53205: Grandjean V, et al. Antimicrobial protection of the mouse testis: synthesis of defensins of the cryptdin family. Biol. Reprod. 57: 1115-1122, 1997. PubMed: 9369178 53206: Vincent S, et al. Stage-specific expression of the Kit receptor and its ligand (KL) during male gametogenesis in the mouse: a Kit-KL interaction critical for meiosis. Development 125: 4585-4593, 1998. PubMed: 9778516 53207: Vidal F, et al. Gene trap analysis of germ cell signaling to Sertoli cells: NGF-TrkA mediated induction of Fra1 and Fos by post-meiotic germ cells. J. Cell Sci. 114: 435-443, 2001. PubMed: 11148144 |
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15P-1
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