- 询价
- 2026年03月22日
企业认证
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- 询价记录
- 文献和实验
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- 细胞形态：
淋巴样
- 库存：
大量
- 年限：
5 years
- 运输方式：
冻存运输
- 生长状态：
悬浮生长
- 物种来源：
猕猴
- 是否是肿瘤细胞：
0
- 相关疾病：
淋巴瘤
- ATCC Number：
CRL-1805™
- 细胞类型：
B淋巴细胞
| Designations: | LCL 8664 | ||
| Depositors: | SR Rangan | ||
| Biosafety Level: | 2 [Cells Contain HERPESVIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Macaca mulatta | ||
| Morphology: | lymphoblast |
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| Source: | Disease: lymphoma Cell Type: B lymphocyte; |
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| Cellular Products: | B lymphotropic herpesvirus | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | 5 years | ||
| Gender: | male | ||
| Comments: | CAUTION: the cells are infected with and produce a B lymphotropic herpesvirus (RhEBV) and may represent a potential biohazard. Free RhEBV is present in the supernatant, and rhesus B lymphocyte transforming activity is present in the medium. About 1% of the cells express virus capsid antigen, and all express the virus related nuclear antigen. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 2 to 3 times per week Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp5 cells/ml and maintain between 4 X 10 exp5 and 2 X 10 exp6 cells/ml. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 22860: Rangan SR, et al. Epstein-Barr virus-related herpesvirus from a rhesus monkey (Macaca mulatta) with malignant lymphoma. Int. J. Cancer 38: 425-432, 1986. PubMed: 3017870 | ||
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文献和实验Production of Human Monoclonal Antibodies by the EpsteinBarr Virus Method
Epstein–Barr virus (EBV) is a herpes virus which in vitro efficiently immortalizes nearly all human B lymphocytes. The lymphoblastoid diploid cell lines (LCL’s) thus generated preserve the characteristics of the cells initially infected
图 UCL（Upper contor limit）=x+A2R=73.25+1.88*2.85=78.10 LCL（Lower contor limit）=x-A2R=67.89 CL（Center limit）=x=73.25 R控制图 UCL=D4R=3.27*2.85=9.32 LCL=D3R=0 Cl=R=2.85 （n为每批检测时应用同浓度质控品的个数） 步骤4.根据一表1及步骤3提供
analysis of human lymphoblastoid cell lines (LCL) for relative quantitation of mitochondrial-localized fluorescent dye intensity. The specific dyes described include MitoTracker Green FM to assess mitochondrial content, tetramethylrhodamine ethyl ester
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