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- 2026年07月09日
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- 文献和实验
- 技术资料
- 物种来源：
大鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 库存：
大量
- 免疫类型：
IgG2b; kappa light chain
- 细胞形态：
淋巴样
- ATCC Number：
HB-326™
- 生长状态：
混合型生长
| Designations: | 15C6 | ||
| Depositors: | A Bendelac | ||
| Isotype: | IgG2b; kappa light chain | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | mixed: adherent and suspension, The cells are rather adherent in nature, and grow best if maintained in suspension culture plates (e.g., Corning Catalog number 430591). | ||
| Organism: | Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against mouse CD1.1 | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with C57BL/6 thymocytes, boosted three times with the RBL-CD1.1 rat cell line (CD1.1-transfected), boosted once with BALB/cJ thymocytes and finally with the CR1-CD1.1 human cell line (CD1.1-transfected). Spleen cells were fused with Sp2/0-Ag14 cells. The antibody blocks recognition of CD1.1 by some, but not all T cells. The antibody reacts with CD1.1, and cross-reacts with CD1.2. The epitope recognized by this antibody is distinct from that recognized by the cluster represented by 15F7 (ATCC HB-322 ) and 20H2 (ATCC HB-323 ). The antibody performs well in flow cytometry applications. |
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| Propagation: | ATCC complete growth medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 95%; fetal bovine serum, 5% Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases. Maintain cultures at cell concentrations between 4 X 10(5) and 8 X 10(5) viable cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 38507: Roark JH, et al. CD1.1 expression by mouse antigen-presenting cells and marginal zone B cells. J. Immunol. 160: 3121-3127, 1998. PubMed: 9531266 38508: Park SH, et al. Tissue-specific recognition of mouse CD1 molecules. J. Immunol. 160: 3128-3134, 1998. PubMed: 9531267 38509: Park SH, et al. Innate and adaptive functions of the CD1 pathway of antigen presentation. Semin. Immunol. 10: 391-398, 1998. PubMed: 9799714 |
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