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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 器官来源：
脾
- 运输方式：
冻存运输
- 品系：
BALB/c
- 生长状态：
悬浮生长
- 年限：
adult
- 免疫类型：
mouse IgG1 kappa
- 细胞形态：
淋巴样
- 库存：
大量
- ATCC Number：
HB-2™
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
| Designations: | T3-3A1 [3A1] | ||
| Depositors: | A Fauci, BF Haynes | ||
| Isotype: | mouse IgG1 kappa | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus | ||
| Morphology: | lymphoblast |
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| Source: | Organ: spleen Strain: BALB/c Cell Type: hybridoma: B lymphocyte; somatic cell hybrid |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | adult | ||
| Comments: | The antibody recognizes a 40000 dalton cell surface antigen that is an early marker of the T cell lineage and may be useful for identifying T cell acute lymphoblastic leukemia. This line is derived from the same fusion used to produce ATCC HB-21 and ATCC HB-22 . |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) cells/ml Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 908: Haynes BF, et al. Human lymphocyte antigens: production of a monoclonal antibody that defines functional thymus-derived lymphocyte subsets. Proc. Natl. Acad. Sci. USA 76: 5829-5833, 1979. PubMed: 316538 916: Eisenbarth GS, et al. Production of monoclonal antibodies reacting with peripheral blood mononuclear cell surface differentiation antigens. J. Immunol. 124: 1237-1244, 1980. PubMed: 6153676 949: Haynes BF, et al. Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans. Proc. Natl. Acad. Sci. USA 77: 2914-2918, 1980. PubMed: 6156457 32301: Prieyl JA, LeBien TW. Interleukin 7 independent development of human B cells. Proc. Natl. Acad. Sci. USA 93: 10348-10353, 1996. PubMed: 8816803 |
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文献和实验指 G． J． Todaro等从 Swiss系小鼠胎儿里得到的细胞株。由于它显示出强烈的接触抑制作用，所以能明确地识别已发生转化的细胞，并作为一种重要的细胞材料使用于由肿瘤病毒和致癌剂等所引起的体外致癌研究。这一细胞系是将 3× 10 5细胞接种在底部直径为 5厘米的培养皿上，是根据每三天进行一次连续培养的方法而建立的。 3T3这一名称便由此而得。同样，由 6× 10 5细胞或 12× 10 5细胞进行接种而得的细胞株，分别称为 3T6和 3T12，这些细胞的接触抑制作用很弱
一光年 最近要做生殖的细胞方面的实验，前期一直用在垂体细胞原代培养，但是一直困扰没有促性腺激素细胞系，但是在网络中搜索，发现老外的文章中经常提到αT3-1，LβT2 或LβT4细胞系，在ATCC等知名的细胞库也未发现这些细胞系，不知道各位高手指点在哪里可以找到这几种细胞系？不甚感激！！ veimojie 我一直也在做这方面的研究 什么地方能有啊 ？ banana_smile 我也想做这方
The cytotoxic effect of chemicals on Balb/c 3T3 cells in culture is measured by cell viability (Neutral Red Uptake) and total cell protein (Kenacid Blue R dye binding method). Healthy Balb/c 3T3 cells (an established cell line
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