- 详细信息
- 文献和实验
- 技术资料
- 生长状态:
悬浮生长
- 免疫类型:
IgG3
- 细胞形态:
淋巴样
- 库存:
大量
- ATCC Number:
HB-8322™
- 物种来源:
小鼠
- 是否是肿瘤细胞:
0
- 运输方式:
冻存运输
| Designations: | 151-5-G2-12 [CO-512, CO512] | ||
| Depositors: | M Herlyn | ||
| Isotype: | IgG3 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
||
| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human Lewis a and Lewis b blood group antigens | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. |
||
| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
||
| Preservation: | 50% FBS+ 40%Culture Medium+10% DMSO | ||
| References: | 1099: Blaszczyk M, et al. Lewis blood group antigens defined by monoclonal anti-colon carcinoma antibodies. Arch. Biochem. Biophys. 233: 161-168, 1984. PubMed: 6465894 | ||
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文献和实验zaiju512 请问有没有人知道哪能买到所有TLRs敲除的小鼠或大鼠? zaiju512 想法有点天真呵呵 hzyyy zaiju512 wrote: 想法有点天真呵呵 文献上有报道的!C3H/Hej小鼠,去上海斯莱克问一下吧! hzyyy 补充一下,C3H/Hej小鼠是TLR4抑制的小鼠,但所有TLR
得上你 kevinddd 我也需要啊! polo5352877 谢谢,你能把你们实验室做那些与癌相关的基因告诉我吗? eggplant512 qinvicky wrote: 我们实验室做基因转染的,一直用N2质粒,不过我懂得不是很多~不知能否帮得上你 我对这个质粒很感兴趣,可否赠我一些呢,我是天津科技大学的研究生。谢谢。万分感谢
zaiju512 求Ca2+振荡产生Ca2+波通过缝隙连接传递的彩图!!谢谢!! zaiju512 急需!!! zaiju512 自己顶起! 本文由丁香园论坛提供,想了解更多有用的、有意思的前沿资讯以及酷炫的实验方法的你,都可以成为师兄的好伙伴 师兄微信号:shixiongcoming
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