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- 2025年11月02日
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- 组织来源：
smooth muscle
- 生长状态：
贴壁生长
- 相关疾病：
正常
- ATCC Number：
CRL-1999™
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 库存：
大量
- 细胞形态：
成纤维样
- 年限：
11 months
- 器官来源：
大动脉
| Designations: | T/G HA-VSMC | ||
| Depositors: | AF Tilberg and RRM Gifford | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: aorta Tissue: smooth muscle Disease: normal |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Reverse Transcript: | N | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 12 D5S818: 12,13 D7S820: 9,10 THO1: 7,8 TPOX: 8 vWA: 16 |
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| Cytogenetic Analysis: | This is a normal human cell line with a 46,XX karyotype. The modal chromosome number was 46, occurring in 90% of cells. The rate of polyploid cells was 0.8%. No structurally altered chromosomes were detected in 15 metaphases karyotyped. Both X chromosomes appeared normal. | ||
| Age: | 11 months | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| HeLa Markers: | N | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.05 mg/ml ascorbic acid; 0.01 mg/ml insulin; 0.01 mg/ml transferrin; 10 ng/ml sodium selenite; 0.03 mg/ml Endothelial Cell Growth Supplement (ECGS); fetal bovine serum to a final concentration of 10%, HEPES to a final concentration of 10 mM, TES to a final concentration of 10 mM. Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, and incubate at 37C for 5 minutes. Add an equal volume of fresh medium, disperse the cells and centrifuge to pellet the cells. Resuspend the cells in fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004 recommended serum:ATCC 30-2020 |
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文献和实验培养了快一个月的细胞了，今天终于鉴定出是我想要的血管平滑肌细胞。我养的是大鼠胸主动脉血管平滑肌细胞,采用的是组织贴块法培养。10天后传第一代（图1），16天后第二代（图2），22天后第三代（图3），并做a－actin免疫细胞化学鉴定（图4）。 图1图2图3 图4典型的血管平滑肌细胞生长"hill and valley"现象。
To fix, 1/10 volume of 37 formaldehyde is added to the culture, which is shaken for a further 40 minutes at room temperature. Cells are then recovered by centrifugation at 1400 X g for 2 minutes, and washed (i.e. gently resuspended then recovered
of 0.75 to 1. To fix, 1/10 volume of 37% formaldehyde is added to the culture, which is shaken for a further 40 minutes at room temperature. Cells are then recovered by centrifugation at 1400 X g for 2 minutes, and washed (i.e. gently resuspended
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