- 详细信息
- 文献和实验
- 技术资料
- 物种来源:
小鼠
- 是否是肿瘤细胞:
0
- 免疫类型:
IgG1
- 细胞形态:
淋巴样
- ATCC Number:
HB-11103™
- 生长状态:
悬浮生长
- 运输方式:
冻存运输
- 库存:
大量
| Designations: | 10D2F6 | ||
| Depositors: | The United States of America | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against an antigen expressed on activated human T cells | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with DS-1 cells (see ATCC CRL-11102 ). Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. The antibody reacts with an antigen expressed on antigen stimulated human T cells and some T cell derived human cell lines. |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine, 50 units/ml penicillin and 0.05 mg/ml streptomycin, 90%; fetal bovine serum, 10% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 2 X 10 exp6 cells/ml. |
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| Preservation: | fetal bovine serum, 97%; DMSO, 3% | ||
| References: | 22116: Bock GH, et al. Monoclonal antibody recognizing a surface molecule on a subset of antigen-stimulated T cells and on certain malignancies of T and B cell origin. US Patent 5,556,947 dated Sep 17 1996 | ||
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文献和实验Early development of primary motor neurons and somites in Zebrafish Embryos
hour stage,the technique of whole mount antibody staining will be used. The ZNP-1 antibody will specifically bind to the primary motor neurons. Since ZNP-1 will not bind to the somites, another antibody,F6,will be used to bind to the somite
就是 NoteFirst 的文献追踪功能啦;RSS 订阅的时候,你需要知道就是 RSS 的 URL;这个也很简单,只要你找到对应期刊的 RSS 订阅页面,举个例子,nature 上面的期刊吧;首先找到期刊的页面;期刊页面的最右侧,看到 RSS feed 了吗,点进去;没错,下面的这些网页还是可以继续点击;找到想要追踪的期刊,点击进入,然后鼠标右键,【查看源代码】即可;在 RSS 的 URL 地址中输入,即可以完成对应的期刊的追踪;然后就是下载的文献管理,有不同的分类可以根据自己的喜好进行分类啦;AkD1f6ZfSg
器来保护。 在无风沙、低污染地区,F7过滤器前可不设预过滤器;在城市中央空调系统中,G3~F6是常见的初级过滤器。 究竟应设什么效率级别的预过滤器来保护后一级过滤器,这需要设计师和现场工程师将使用环境、备件费用、运行能耗、维护费用等因素综合考虑后决定。 中央空调过滤器的使用寿命同时还取决于过滤面积和过滤材料的选择。过滤器中滤材的展开面积经常是过滤器迎风面的数倍、数十倍,有时达到一百倍。过滤面积大,能容纳的粉尘就多,过滤器的使用寿命就长。过滤面积大,气流穿过材料的速度就低,过滤器的阻力
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