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- 2025年11月10日
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- 文献和实验
- 技术资料
- 库存：
大量
- 器官来源：
其他
- 运输方式：
冻存运输
- ATCC Number：
CRL-1660™
- 年限：
larva
- 物种来源：
其他
- 是否是肿瘤细胞：
0
- 生长状态：
贴壁生长
| Designations: | Aedes albopictus clone C6/36 | ||
| Depositors: | KH Eckels | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Aedes albopictus | ||
| Morphology: | |
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| Source: | Organ: larva, whole | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Tumorigenic: | No | ||
| Cytogenetic Analysis: | diploid | ||
| Age: | larva | ||
| Comments: | This cell line is useful for the replication of flaviviruses and reportedly can be used to replicate Dengue viruses to high titers. The cells are non-anchorage dependent, are not tumorigenic and maintain a diploid chromosome number. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 28.0°C |
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| Subculturing: | Protocol: Subcultures are prepared by scraping or by vigorous pipetting. Remove the old medium, add fresh complete culture medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Twice per week |
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| Preservation: | Freeze medium: culture medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 parental cell line:ATCC CCL-126 |
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| References: | 22502: Igarashi A. Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses. J. Gen. Virol. 40: 531-544, 1978. PubMed: 690610 22812: Singh KRP. Cell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.). Curr. Sci. 36: 506-508, 1967. |
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文献和实验Development of an In Vitro mRNA Decay System in Insect Cells
Cytoplasmic extracts have proven to be a versatile system for assaying the mechanisms and interactions of RNA metabolism. Using Aedes albopictus (C6/36) cells adapted to suspension culture, we have been able to faithfully reproduce and manipulate
|998894|gb|S78179.1|S78179 Oryctolagus cuniculus type XII... 32 0.037 gi|31072033|gb|AC122153.4| Oryctolagus cuniculus clone LB1-... 28 0.58 gi|1496|emb|X52496.1|OCCAATP Rabbit mRNA for calcium ATPase 28 0.58 gi|1468|emb|X02814.1|OCATPDR Rabbit mRNA
下面是我设计的引物的BLAST结果，请大虾帮我解释一下。谢谢
.1|S57971 Rh polypeptide II {clone RhPII... 36 3.4 gi|36027|emb|X63094.1|HSRHII H.sapiens mRNA for rhesus poly... 36 3.4 gi|18307511|emb|AJ428455.1|HSA428455 Homo sapiens partial R... 36 3.4 gi|606991|gb|L37050.1|CHPRHC Pan troglodytes (clone 317-IIR
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