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- 技术资料
- 器官来源：
其他
- 物种来源：
其他
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 年限：
larva
- ATCC Number：
CCL-126™
- 细胞形态：
上皮样
- 库存：
大量
- 生长状态：
贴壁生长
| Designations: | Aedes albopictus (Mosquito larva) | ||
| Depositors: | SM Buckley | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Aedes albopictus | ||
| Morphology: | epithelial |
||
| Source: | Organ: larva | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Virus Susceptibility: | Dengue virus type 1 Dengue virus type 2 Dengue virus type 3 Colorado tick fever virus Dengue virus type 4 Japanese encephalitis virus Human poliovirus 1 Human herpesvirus 1 Vaccinia virus Vesicular stomatitis virus |
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| Cytogenetic Analysis: | Karyotype stable within diploid stemline number. The chromosomes have median centromeres. In a few cases, a chromosome with a submedian centromere was noted. Two (2) cells with chromosome breaks (3%) and 6 cells with secondary constrictions (10%). | ||
| Age: | larva | ||
| Propagation: | ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 1.0 mM sodium pyruvate, 80%; heat-inactivated fetal bovine serum, 20% Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 28.0°C; (Max. 28.0 °C, Min. 26.0°C) |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 1 to 2 times per week |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 26 | ||
| Related Products: | recommended serum:ATCC 30-2020 derivative:ATCC CRL-1660 |
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| References: | 22812: Singh KRP. Cell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.). Curr. Sci. 36: 506-508, 1967. 26288: Singh KRP, Paul SD. Multiplication of arboviruses in cell lines from from Aedes albopictus and Aedes aegypti. Curr. Sci. 37: 65-67, 1968. 26289: Singh KR. Propagation of arboviruses in Singh's Aedes cell lines. I. Growth of arboviruses in Aedes albopictus and A. aegypti cell lines. Curr. Top. Microbiol. Immunol. 55: 127-133, 1971. PubMed: 5142320 26291: . . Contrib. Boyce Thompson Inst. 22: 435-460, 1964. 26292: Buckley SM. Susceptibility of the Aedes albopictus and A. aegypti cell lines to infection with arboviruses. Proc. Soc. Exp. Biol. Med. 131: 625-630, 1969. PubMed: 5787147 26295: Yunker CE, Cory J. Colorado tick fever virus: growth in a mosquito cell line. J. Virol. 3: 631-632, 1969. PubMed: 5798247 |
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