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- 技术资料
- ATCC Number：
CRL-2797™
- 组织来源：
smooth muscle
- 品系：
C57BL/6
- 器官来源：
大动脉
- 库存：
大量
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
- 细胞形态：
其他
- 年限：
adult
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
| Designations: | MOVAS | ||
| Depositors: | M Husain | ||
| Biosafety Level: | 2 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | spindle-shaped |
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| Source: | Organ: aorta Strain: C57BL/6 Tissue: smooth muscle Cell Type: immortalized with SV40 large T antigen |
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| Cellular Products: | actin; smooth muscle alpha actin calponin 1; basic; smooth muscle desmin smooth muscle protein 22 (SM22 alpha) |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: December, 1999 | ||
| Age: | adult | ||
| Comments: | Primary vascular aortic smooth muscle cells isolated by collagenase - elastase digestions in December 1999 were transduced with retrovirus encoding the SV40 large T antigen as well as the gene for neomycin resistance. Clones were selected in the presence of 0.4 mg/ml G418 and subcloned by limiting dilution. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Interval: Maintain cultures at a cell concentration between 1 X 10(4) and 1 X 10(5) cells/sq. cm Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Two to three times weekly |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 15 hours | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 Cell culture tested DMSO:ATCC 4-X Erythrosin B vital stain solution:ATCC 30-2404 |
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| References: | 47767: Afroze T, Husain M. c-Myb-binding sites mediate G(1)/S-associated repression of the plasma membrane Ca(2+)-ATPase-1 promoter. J. Biol. Chem. 275: 9062-9069, 2000. PubMed: 10722757 88855: Afroze T, et al. Calcineurin-independent regulation of plasma membrane Ca2+ ATPase-4 in the vascular smooth muscle cell cycle. Am. J. Physiol. Cell Physiol. : C88-C95, 2003. PubMed: 12660151 |
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