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- 相关疾病：
其他疾病
- ATCC Number：
CRL-8066™
- 细胞形态：
淋巴样
- 生长状态：
悬浮生长
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 库存：
大量
- 细胞类型：
T淋巴细胞
| Designations: | Mo [Mo T] | ||
| Depositors: | Genetics Institute, Inc., Regents of the University of California | ||
| Biosafety Level: | 2 [Cells Contain RETROVIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Disease: hairy cell leukemia Cell Type: T lymphocyte; |
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| Cellular Products: | granulocyte macrophage colony stimulating factor (GM-CSF, GM CSF), also known as neutrophil migration inhibitory factor (NIF-T); interferon | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10 D13S317: 9,13 D16S539: 11 D5S818: 11,12 D7S820: 9,12 THO1: 9.3 TPOX: 8,11 vWA: 17,18 |
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| Gender: | male | ||
| Comments: | A plasmid containing the DNA sequence coding from Mo that codes for CSF is available as ATCC 39754. Mo cells contain a replication competent genome of Human T Cell Leukemia Virus II (HTLV-II) and two defective HTLV-II genomes. For long term culture, the cells can be grown in medium with 2% fetal bovine serum. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Temperature: 37.0°C Atmosphere: 8 to 9% CO2 in air |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended The cells are subcultured every 7 days by centrifugation and division of the cell pellet 1:4 (1:6 maximum). Do not dilute below 2 X 105 viable cells/ml or allow to exceed 2 X 106 cells/ml. Culture at 8 to 9% CO2 in air. The donor suggests using Falcon flasks for optimum growth. |
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| Preservation: | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2005 Recommended serum: ATCC 30-2020 Cell culture tested DMSO: ATCC 4-X Erythrosin B vital stain solution: ATCC 30-2404 |
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| References: | 3527: Golde DW, Quan SG. Unique T-lymphocyte line and products derived therefrom. US Patent 4,438,032 dated Mar 20 1984 22772: Clark SC, et al. Method for identification and isolation of DNA encoding a desired protein. US Patent 4,675,285 dated Jun 23 1987 22828: Saxon A, et al. Immunologic characterization of hairy cell leukemias in continuous culture. J. Immunol. 120: 777-782, 1978. PubMed: 344797 23502: Gasson JC, et al. Purified human granulocyte-macrophage colony-stimulating factor: direct action on neutrophils. Science 226: 1339-1342, 1984. PubMed: 6390681 |
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文献和实验Mechanism of Mo-Dependent Nitrogenase
Nitrogenase is the enzyme responsible for biological reduction of dinitrogen (N2 ) to ammonia, a form usable for life. Playing a central role in the global biogeochemical nitrogen cycle, this enzyme has been the focus of intensive research
只能为整个金属晶格所共有。所以金属键不同于离子键；也不同于共享电子局限在两个原子间的那种共价键 ( 定域键 ) 。广义地说，金属键属于离域键，即共享电子分布在多个原子间的一种键，但它是一种特殊的离域键，既无方向性，也无饱和性。 为阐明金属键的特性，化学家们在 MO 理论的基础上，提出了能带理论。现仅以金属 Li 为例定性讨论 。 Li 原子核外电子为 1s 2 2s 1 。两个 Li 互相靠近形成 Li 2 分子。按照 MO 理论， Li 分子应有四
-CSF。其中T细胞和巨噬细胞一般在免疫应答或炎症介质刺激过程中直接产生；而内皮细胞、成纤维细胞可能通过IL-1和TNF的诱导而产生。 表4-4 CSF的生物学功能及临床应用 CSF 分子量（kDa) 鼠 人 主要产生细 胞 主要集落刺激作用 促进吞噬细胞功能 临床治疗疾病 使用情况 基因定位 外显子/内含子 人与鼠同源性（%） 人与鼠活性交叉 糖基化 G-CSF（CSF-β） 25 30 Mo,En,Fb 髓样、中性粒细胞集落增殖、分化成熟 PMN的吞噬
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