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- 相关疾病：
其他疾病
- ATCC Number：
CCL-245™
- 生长状态：
悬浮生长
- 器官来源：
外周血
- 年限：
33 years
- 运输方式：
冻存运输
- 库存：
大量
- 细胞形态：
淋巴样
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
B淋巴细胞
| Designations: | Mo-B | ||
| Depositors: | DW Golde | ||
| Biosafety Level: | 2 [Cells Contain RETROVIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Organ: peripheral blood Disease: hairy cell leukemia Cell Type: B lymphoblast; |
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| Cellular Products: | cells produce infectious HTLV II | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,13 D13S317: 9,13 D16S539: 11 D5S818: 11,12 D7S820: 9,12 THO1: 9.3 TPOX: 8,11 vWA: 17,18 |
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| Isoenzymes: | ES-D, 1 G6PD, B GLO-I, 2 PGM1, 1 PGM3, 0 |
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| Age: | 33 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The cells produce infectious human T cell leukemia virus and must be handled as a potential biohazard under P-2 conditions. The line is EBNA positive (EBNA+). | ||
| Propagation: | ATCC complete growth medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20% |
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| Subculturing: | Protocol: The cells are subcultured every 7 days by centrifugation .For optimum results do not culture below 8 X 10(5 ) cells/ml or above 2 X 10(6) cells/ml. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: none |
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| References: | 1053: Chen IS, et al. Human T-cell leukemia virus type-II transforms normal human lymphocytes. Proc. Natl. Acad. Sci. USA 80: 7006-7009, 1983. PubMed: 6316341 26068: Saxon A, et al. T-lymphocyte variant of hairy-cell leukemia. Ann. Intern. Med. 88: 323-326, 1978. PubMed: 305224 37428: Chen IS, et al. Molecular characterization of genome of a novel human T-cell leukaemia virus. Nature 305: 502-505, 1983. PubMed: 6312323 |
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文献和实验相关实验
Mechanism of Mo-Dependent Nitrogenase
Nitrogenase is the enzyme responsible for biological reduction of dinitrogen (N2 ) to ammonia, a form usable for life. Playing a central role in the global biogeochemical nitrogen cycle, this enzyme has been the focus of intensive research
化，其他已知细胞松弛素 B还可抑制糖的输送，可以考虑细胞松弛素 B与细胞膜间的相互作用。此外还分离出数种具有类似结构和功能的物质，分别命名为细胞松弛素 A、 C、 D、 E、 F。
细胞色素b563 cytochrome b563 亦称细胞色素 b6 。存在于高等植物至藻类的叶绿体中，与类囊体膜结合紧密。含有原正铁血红素，氧化还原 （β带）和 434nm（γ带或 So－ ret带）。为发动光合磷酸化的环状电子传递系统的一个成分，可被光化系统Ⅰ所还原和氧化。
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