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- 2025年11月23日
企业认证
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- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 库存：
大量
- 运输方式：
冻存运输
- 细胞形态：
淋巴样
- 相关疾病：
其他疾病
- ATCC Number：
CRL-3000™
- 生长状态：
悬浮生长
- 器官来源：
外周血
- 组织来源：
Mantle cell lymphoma (B cell non-Hodgkin's lymphoma)
- 年限：
64
| Designations: | Mino | ||
| Depositors: | R.J.Ford | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast Characterization Data |
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| Source: | Organ: peripheral blood Tissue: Mantle cell lymphoma (B cell non-Hodgkin's lymphoma) Cell type: lymphoblast; cytogenetic abnormality Disease: Mantle Cell Lymphoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | The immunophenotype by flow cytometry is compatible with MCL [PubMed: 12127561]. | ||
| Antigen Expression: | CD3-, CD5+, CD10-, CD19+, CD20+, CD23- (verified at ATCC ) | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 9,11 D13S317: 12 D16S539: 11,12 D5S818: 11,12 D7S820: 10,11 THO1: 9.3 TPOX: 8,11 vWA: 14,17 |
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| Cytogenetic Analysis: | Modal chromosome number: 74. This is a hypertriploid male cell line, del(6)(q16), t(8;11)(q22;q23), add(11)(q25), add(13)(q34), add(14)(q32). Most cells contain the t(11;14) derivative chromosome, detected by FISH with whole chromosome paint probes. | ||
| Age: | 64 | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The cells are large, growing singly and in small clumps in vitro. The immunophenotype by flow cytometry is compatible with MCL [PubMed: 12127561].Western blots show expression of cyclin D1 but no detectable cyclin D2 and cyclin D3; the retinoblastoma protein is predominantly phosphorylated. There is expression of tumor suppressor gene products including p53, p16 (INK4a), and p21(WAF1) [PubMed: 12127561]. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 6 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 1.5 X 10(6) cells/ml. Subcultivation Ratio: 1:5 to 1:10. | ||
| Preservation: | 90% FBS; 10% (v/v) DMSO | ||
| Doubling Time: | about 72 hours | ||
| References: | 92889: Lai R, et al. Establishment and characterization of a new mantle cell lymphoma cell line, Mino. Leuk. Res. 26: 849-855, 2002. PubMed: 12127561 92890: Marzec M, et al. Mantle cell lymphoma cells express predominantly cyclin D1a isoform and are highly sensitive to selective inhibition of CDK4 kinase activity. Blood 108: 1744-1750, 2006. PubMed: 16690963 92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365 |
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文献和实验SILAC-Based Quantification of Sirt1-Responsive Lysine Acetylome
S table I sotope L abeling by A mino acids in C ell culture (SILAC) is one of the in vivo metabolic labeling methods widely used for dynamic analysis of protein modifications. Here, we describe a general approach to applying SILAC
叶枕 pulvinus， leaf cushion 亦称叶褥。（ 1）种子植物在叶柄下端或上端产生的关节样肥厚部分，称之为叶枕。含羞草（ Mino-sa pudica）的叶片受到刺激时，叶枕细胞的渗透压升高，产生活动电位，而引起向性运动。此外，豆科（ Leguminosae）、酢浆草科（ Oxalidaceae）、薯蕨科（ Dioscareaceae）等由于细胞吸水膨压的变化，常朝向日光的方向，进行叶片的变位运动或睡眠运动。（ 2）石炭纪化石蕨类的封印木（ Sigillaria）及鳞木
正常功能有重要意义。例如人或动物进食山藜豆所致的山藜豆中毒(lathyrivsm)表现为严重的骨、关节及大血管壁的异常，其发病即因为山藜豆毒素β―氨基丙晴(β―mino propionitrile) 与赖氨酸氧化酶活性位点共价结合而使之失活，从而导致胶原纤维变脆弱。 胶原蛋白与许多人类疾病相关。如骨形成不全(osteogenesis imperfecta)或称脆骨病(brittle bone diesease)，即是由于胶原蛋白生物合成或转录后的修饰作用障碍所致。现已清楚
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