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- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
内皮细胞
- 器官来源：
其他
- 细胞形态：
内皮样
- 组织来源：
vascular endothelium
- 库存：
大量
- 生长状态：
贴壁生长
- 相关疾病：
正常
- ATCC Number：
CRL-1730™
- 运输方式：
冻存运输
| Designations: | HUV-EC-C | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | endothelial |
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| Source: | Organ: umbilical vein Tissue: vascular endothelium Disease: normal Cell Type: endothelial |
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| Cellular Products: | factor VIII [23284 ] | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Tumorigenic: | No | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11,12 D13S317: 9,11 D16S539: 11,12 D5S818: 11,12 D7S820: 8,12 THO1: 6,9.3 TPOX: 8,11 vWA: 16 |
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| Cytogenetic Analysis: | Karyology performed for one batch of CRL-1730 in 1996 reflected a hypodiploid human cell line with a modal chromosome number of 45 occurring in 72% of the cells counted, all of which had monosomic N13. The rate of polyploid cells among this population was 15.8%. This karyology differed from earlier work-ups performed on the cells that showed approximately 60% of the cells retained 2 chromosomes 13. The apparent clonal variation in cultures of CRL-1730 (most likely dependent upon passage and growth conditions) has also been noted in STR profiles with unstable alleles at D13S317 allele #9, D13S317 allele #11, and D7S820 allele #12. Other coexisting subclones include those with 46,XX,-11,-13,i(11p),i(11q) and 46,XX,+11,-13 karyotypes. For all karyotypes performed, both X chromosomes appear normal. | ||
| Comments: | Endothelial Cell Growth Supplement (ECGS) and unidentified factors from bovine pituitary, hypothalamus or whole brain extracts are mitogenic for this line. [23284 ] The cells have a life expectancy of 50 to 60 population doublings. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated of F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.1 mg/ml heparin; 0.03-0.05 mg/ml endothelial cell growth supplement (ECGS); adjust to a final concentration of 10% fetal bovine serum. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Note: A high quality ECGS prepared from bovine neural tissue (Sigma Cat no. E-2759 or equivalent) should be used to propagate CRL-1730. It is best to initiate the cells with the highest recommended concentration of ECGS.Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Two to three times per week |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004 recommended serum:ATCC 30-2020 |
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| References: | 23284: Hoshi H, McKeehan WL. Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture. Proc. Natl. Acad. Sci. USA 81: 6413-6417, 1984. PubMed: 6333682 29192: Zahedi K. Characterization of the binding of serum amyloid P to laminin. J. Biol. Chem. 272: 2143-2148, 1997. PubMed: 8999915 33021: Soker S, et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996. PubMed: 8621443 |
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HUV-EC-C
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