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- 详细信息
- 技术资料
- 运输方式：
冻存运输
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- 细胞类型：
成纤维细胞
- ATCC Number：
SCRC-1040.2a™
- 生长状态：
贴壁生长
- 细胞形态：
成纤维样
- 器官来源：
胚胎
- 年限：
14 days gestation embryo
| Designations: | MEF (CF-1) MITC | ||
| Depositors: | ATCC | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
||
| Source: | Organ: embryo Cell Type: fibroblast |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 2004 | ||
| Applications: | Mitomycin-C treated cells for use as feeder layer | ||
| Age: | 14 days gestation embryo | ||
| Gender: | male and female mixed | ||
| Comments: | These cells are provided for use as feeder cells to support the growth of stem cells in the undifferentiated state. They have been treated with Mitomycin C at passage #3 and will not replicate. The cells will begin to deteriorate 7 days after plating. Once the feeder cells have attached, the culture medium can be changed to accommodate the cells to be supported. It is recommended that the feeder cells be plated 24 hours before use at 5 to 6 X 10(6)/T75 in order to obtain a 100% confluent monolayer for stem cell growth. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Establishing and maintaining your culture: To insure the highest level of viability, be sure to warm media to 37�C before using it on the cells. Flasks do not need to be coated before plating MEFs.
Medium Renewal: Twice a week or when pH decreases |
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| Preservation: | Storage temperature: liquid nitrogen vapor phase | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2002 recommended serum: ATCC 30-2020 source culture: ATCC SCRC-1040 |
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| References: | 89421: Nagy A, et al. Manipulating The Mouse Embryo: A Laboratory Manual. Third Edition: Cold Spring Harbor Press; 2003. | ||
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MEF (CF-1) MITC
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