- 询价
- 2025年12月02日
企业认证
- 详细信息
- 文献和实验
- 技术资料
- 生长状态：
悬浮生长
- 库存：
大量
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 免疫类型：
IgG2a
- 细胞形态：
淋巴样
- ATCC Number：
HB-9403™
| Designations: | Hybridoma 234 s.2a | ||
| Depositors: | Wistar Institute | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against canine lymphoma cells | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with canine lymphoma cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts preferentially with canine monocytes, but also reacts with canine lymphocytes. It does not cross-react with human cells. This is an IgG2a switch variant of Hybridoma 234 (see ATCC HB-8402 ). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | fetal bovine serum, 95%; DMSO, 5% | ||
| References: | 22010: Steplewski Z, Jeglum KA. Monoclonal antibodies against lymphoma-associated antigens, hybrid cell lines producing these antibodies. US Patent 5,169,775 dated Dec 8 1992 | ||
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文献和实验The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridoma fusion: Immortalization of sensitized B lymphocytes from immune mice. Overview
Fusion voltage Range in hybridoma formation efficiency (5 ) Z10 (18 ) CMV 5 2 �
motifs located prevalently in the 3′ untranslated regions of target messenger RNAs (mRNA). Interferon-alpha-2a (IFNα) induces a large set of protein coding genes mediating antiproliferative and antiviral responses. Here we use a global microarray-based
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