- 详细信息
- 技术资料
- 器官来源:
肺
- 年限:
20 years
- 物种来源:
人
- 是否是肿瘤细胞:
0
- ATCC Number:
CRL-7624™
- 库存:
大量
- 细胞形态:
成纤维样
- 生长状态:
贴壁生长
- 运输方式:
冻存运输
| Designations: | Hs 888.Lu | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: lung | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | 20 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The line was established from apparently normal tissue from a patient who had metastatic osteosarcoma (see ATCC CRL-7622 ). See also ATCC CRL-7517 CRL-7632, ATCC CRL-7633, ATCC CRL-7634, ATCC CRL-7635, ATCC CRL-7642, ATCC CRL-7643, ATCC CRL-7644 and ATCC CRL-7645 for lines derived from the same patient. Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. Please see the NBL Repository description. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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Hs 888.Lu
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