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        Liquid Chromatographic Method for Aflatoxin M1 in Milk

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        Aflatoxin M1 is the 4-hydroxy derivative of aflatoxin B1 (see Fig. 1 ). Aflatoxin M1 appears in milk and milk products as the direct result of the intake of aflatoxin B1 -contaminated feed by dairy cows (1 ). The amount excreted as aflatoxin M1 , as a percentage of aflatoxin B1 in feed, is usually 1-4% (2 ), but values as high as 6% have been reported at μg daily intake levels of aflatoxin B1 (3 ). The carry-over of aflatoxin B1 to milk may vary largely from animal to animal, from day to day, and from one milking to the next.
        http://img.dxycdn.com/trademd/upload/asset/meeting/2014/02/14/B1392271951.jpg
        Fig. 1.  Chemical structure of aflatoxin M1 .

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