BALB/3T3 clone A31

【ATCC来源】BALB/3T3 clone A31、BALB/3T3 clone A31、BALB/3T3 clone A31细胞、BALB/3T3 clone A31细胞、BALB/3T3 clone A31BALB/3T3 clone A31[小鼠胚胎成纤维细胞]

Cell line name BALB/3T3 clone A31

Synonyms BALB/c 3T3 clone A31; Balb/c3T3; BALB/c 3T3; Balb/c 3T3; BALB/3T3; Balb/3T3-4-Cl31; 3T3 clone A31; BALB/3T3 cl. A31; BALB 3T3 clone A31; BALB/3T3 (clone A31); B/C3T3; 3T3-A31; 3T3(A31); A31; A31N

Accession CVCL_0184

Resource Identification Initiative To cite this cell line use: BALB/3T3 clone A31 (RRID:CVCL_0184)

Comments Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).

Doubling time: 30-32 hours (Note=At 33 Celsius) (PubMed=4798834); 24 hours (Note=At 38 Celsius) (PubMed=182373).

Omics: SNP array analysis.

Misspelling: BABL/3T3; KCB=KCB 200713YJ.

Derived from site: In situ; Embryo; UBERON=UBERON_0000922.

Cell type: Fibroblast; CL=CL_0000057.

Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)

Breed/subspecies: BALB/c.

Hierarchy Children:

CVCL_6760 (12-A-3) CVCL_VT75 (17-Cl1) CVCL_A6ZR (3T3/40L)

CVCL_VI61 (A31-714) CVCL_A9DY (A31-IS11 EGF-2) CVCL_LF96 (B77-A31)

CVCL_B5VL (BALB/3T3 clone A31 H) CVCL_B5VH (BALB/3T3 clone A31 NTG2) CVCL_B5VD (BALB/3T3 clone A31 Ts-1)

CVCL_B5VE (BALB/3T3 clone A31 ts2) CVCL_B385 (BALB/3T3 clone A31 ts20) CVCL_B5VG (BALB/3T3 clone A31 ts22)

CVCL_A5PN (BALB/3T3 clone A31T) CVCL_4350 (K-BALB) CVCL_5793 (M-MSV-BALB/3T3)

CVCL_LF97 (MC5-5) CVCL_U900 (MT-5) CVCL_T375 (RGB3T3-1)

CVCL_T376 (RGB3T3-5) CVCL_4554 (SV-T2) CVCL_6462 (UC1B)

Sex of cell Sex unspecified

Age at sampling 14-17FD

Category Spontaneously immortalized cell line

STR profile Source(s): PubMed=31220119

 

Markers:

Mouse STR 1-1 16

Mouse STR 1-2 17

Mouse STR 2-1 16

Mouse STR 3-2 14

Mouse STR 4-2 21.3

Mouse STR 5-5 14

Mouse STR 6-4 18

Mouse STR 6-7 12

Mouse STR 7-1 25.2

Mouse STR 8-1 13

Mouse STR 11-2 17

Mouse STR 12-1 16

Mouse STR 13-1 16.2

Mouse STR 15-3 22.3

Mouse STR 17-2 15,16

Mouse STR 18-3 18

Mouse STR 19-2 14

Mouse STR X-1 25

 

Run an STR similarity search on this cell line

Publications

PubMed=4301006; DOI=10.1002/jcp.1040720208

Aaronson S.A., Todaro G.J.

Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40.

J. Cell. Physiol. 72:141-148(1968)

 

PubMed=4359949; DOI=10.1128/JVI.12.4.711-720.1973; PMCID=PMC356689

Benveniste R.E., Todaro G.J., Scolnick E.M., Parks W.P.

Partial transcription of murine type C viral genomes in BALB c cell lines.

J. Virol. 12:711-720(1973)

 

PubMed=4792350; DOI=10.1002/ijc.2910120217

Kakunaga T.

A quantitative system for assay of malignant transformation by chemical carcinogens using a clone derived from BALB-3T3.

Int. J. Cancer 12:463-473(1973)

 

PubMed=4798834; DOI=10.1016/0014-4827(73)90283-8

Wittes R.E., Ozer H.L.

Characterization of a temperature-sensitive mutant of BALB/c 3T3 cells.

Exp. Cell Res. 80:127-136(1973)

 

PubMed=50878

Quarles J.M., Tennant R.W.

Effects of nitrosocarbaryl on BALB/3T3 cells.

Cancer Res. 35:2637-2643(1975)

 

PubMed=182373; DOI=10.1016/0092-8674(76)90028-3

Slater M.L., Ozer H.L.

Temperature-sensitive mutants of Balb/3T3 cells: description of a mutant affected in cellular and polyoma virus DNA synthesis.

Cell 7:289-295(1976)

 

PubMed=10848989; DOI=10.1046/j.1432-1327.2000.01404.x

Salvat C., Acquaviva C., Scheffner M., Robbins I., Piechaczyk M., Jariel-Encontre I.

Molecular characterization of the thermosensitive E1 ubiquitin-activating enzyme cell mutant A31N-ts20. Requirements upon different levels of E1 for the ubiquitination/degradation of the various protein substrates in vivo.

Eur. J. Biochem. 267:3712-3722(2000)

 

PubMed=22576795; DOI=10.3892/or.2012.1763

Ashinuma H., Takiguchi Y., Kitazono S., Kitazono-Saitoh M., Kitamura A., Chiba T., Tada Y., Kurosu K., Sakaida E., Sekine I., Tanabe N., Iwama A., Yokosuka O., Tatsumi K.

Antiproliferative action of metformin in human lung cancer cell lines.

Oncol. Rep. 28:8-14(2012)

 

PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738

Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. 3rd, Pardo-Manuel de Villena F.

SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.

BMC Genomics 15:847.1-847.11(2014)

 

PubMed=31220119; DOI=10.1371/journal.pone.0218412; PMCID=PMC6586308

Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B.A., Gollin S.M., Lewis D.W., Reid Y.A.

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.

PLoS ONE 14:E0218412-E0218412(2019)