- 详细信息
- 文献和实验
- 技术资料
- 相关疾病:
正常
- ATCC Number:
CRL-1926™
- 细胞形态:
成纤维样
- 运输方式:
冻存运输
- 年限:
embryo
- 物种来源:
其他
- 是否是肿瘤细胞:
0
- 库存:
大量
- 生长状态:
贴壁生长
- 器官来源:
胚胎
| Designations: | NZP-46 | ||
| Depositors: | L Munson | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Calloscirus notatus | ||
| Morphology: | fibroblast |
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| Source: | Organ: embryo Disease: normal |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | embryo | ||
| Comments: | The cells can be propagated for at least 17 population doublings beyond frozen reference seed stock. | ||
| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium, 45%; Ham's F12 medium, 45%; fetal bovine serum, 10%; plus the following additives: 10 ng/ml epidermal growth factor; 0.005 mg/ml insulin; 5 ng/ml selenium; 0.005 mg/ml transferrin |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the flask to sit at room temperature, or incubate at 37C, until the cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
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文献和实验CD46 分子 CD46 常用单克隆抗体或代号: HULYM5,J48 主要表达细胞: 广泛,Leu,Pt [NL] 分子质量(kDa)和结构: gp56- 66,膜辅助因子蛋白(MCP) 功 能: 调节补体活化,裂解C3b ,C4b膜辅助因子蛋白 CD46 Aka membrane cofactor protein (MCP) Cofactor for Factor
【求助】质粒有两个复制起始点,怎么复制呢?(red重组,pKD46质粒)
mnidr 我现在在做基因敲除,用的是red重组系统,pKD46质粒上携带有敲除所需的蛋白。pKD46质粒上有两个复制起始点Origin of Replication:repA101ts & oriR101 ;repA101ts是温度敏感型复制起始点。 有几个问题请教: 1.大家有用过这个pKD46的吗?oriR101是温度敏感型复制子还是条件诱导型复制子啊,我在网上查到它是温度敏感型复制起始点,我很不理解,为什么已经有一个温度
网络 四、膜辅蛋白(CD46) 膜辅蛋白(membrane cofactor protein,MCP)由Cole等(1985)应用C3b亲和层析在外周血淋巴细胞上发现的一种膜蛋白。由于其奇特的电泳特征,起初被命名为gp45-70,但进一步研究发现,它对I因子介导的对C3b和C4b的裂解有辅助活性,故更命为MCP。鉴于MCP
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