- 询价
- 2025年12月05日
14 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 技术资料
- 年限：
74 years
- 生长状态：
贴壁生长
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 库存：
大量
- ATCC Number：
HTB-125™
- 细胞形态：
成纤维样
- 器官来源：
乳房
| Designations: | Hs 578Bst | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
||
| Source: | Organ: mammary gland; breast | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Receptors: | epidermal growth factor (EGF) | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11,13 D13S317: 11,13 D16S539: 9,12 D5S818: 11,13 D7S820: 10 THO1: 9,9.3 TPOX: 8 vWA: 17 |
||
| Cytogenetic Analysis: | modal number = 46; range = 42 to 48.. This is a diploid human cell line with 46,XX karyotype. Polyploidy occurred at 6.9%. Both X chromosomes were normal. The chromosome N9 pair was heteromorphic for the centromeric heterochromatin having one with the normal size and the other about twice the size of the normal. |
||
| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 0 PGM1, 1 PGM3, 1 |
||
| Age: | 74 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | Hs 578Bst was derived by A.J. Hackett, et al. from normal breast tissue peripheral to an infiltrating ductal carcinoma which was the source for Hs 578T (see ATCC HTB-126 ). Hs 578Bst may have been myoepithelial in origin since the cells possessed microfilaments and clusters of pinocytotic vesicles similar to those seen in myoepithelia in vivo. No desmosomes were observed, estrogen receptor protein was not present, and no endogenous viruses were detected. Note: A frozen ampule at unknown population doubling (PDL) was received at the ATCC in 1983. Cells had the potential to reach approximately 22 more population doublings before the onset of senescence. See Batch Specific information for PDL of current Distribution freeze. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: 30 ng/ml mouse EGF; fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
||
| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: 2 to 3 times per week |
||
| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
||
| Related Products: | recommended serum:ATCC 30-2020 Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X tumor cell line established from the same patient:ATCC HTB-126 |
||
| References: | 1119: Hackett AJ, et al. Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs 578T) and the diploid myoepithelial (Hs 578Bst) cell lines. J. Natl. Cancer Inst. 58: 1795-1806, 1977. PubMed: 864756 21947: Hancock ME, et al. Method for predicting chemosensitivity of anti-cancer drugs. US Patent 4,937,182 dated Jun 26 1990 24344: Stampfer MR, et al. Enhanced growth medium and method for culturing human mammary epithelial cells. US Patent 4,423,145 dated Dec 27 1983 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
技术资料暂无技术资料 索取技术资料
Hs 578Bst
询价
