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- 文献和实验
- 技术资料
- 库存：
大量
- ATCC Number：
HB-257™
- 生长状态：
悬浮生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 免疫类型：
IgG2a
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
| Designations: | FW11-10-3 | ||
| Depositors: | CR Mackay | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
||
| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against the v4 variant of human CD44 | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with a fusion protein corresponding to the variant region of human CD44 (exons 3v to 10v). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for the product of the 4v exon. |
||
| Propagation: | ATCC complete growth medium: Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10% |
||
| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
||
| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 22355: Mackay CR, et al. Expression and modulation of CD44 variant isoforms in humans. J. Cell Biol. 124: 71-82, 1994. PubMed: 7507492 | ||
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文献和实验Construction of cDNA Libraries in gt10 or gt11
that it is possible to divide the mRNA population into three classes with most of the mRNAs (11,300) being in the lowest abundance class and making up 39% of the polysomal mRNA. To obtain a cDNA library that contains at least one clone for each mRNA of this class
不锈钢镊子系列：ST-10，ST-11，ST-12，ST-13，ST-14，
不锈钢镊子系列：ST-10，ST-11，ST-12，ST-13，ST-14，ST-15，ST-16，ST-17，TS-10，TS-11，TS-12，TS-13，TS-14，TS-15，TS-16，ST-17可换头防静电镊子系列：ESD-259，ESD-259A，ESD-250，ESD-249，ESD-242，ESD-7A，ESD-00，ESD-2A，不锈钢精密镊子系列：SS-SA，OO-SA，AA-SA，1-SA，2-SA，2A-SA，3-SA，3C-SA，4-SA，5-SA
10.11 PNAS 揭示生长素调控细胞分裂模式新机制；外泌体 HIV- 1 Tat 对人细胞蛋白表达的影响
① PNAS 揭示生长素调控细胞分裂模式新机制细胞分裂模式在器官的形成过程中发挥着至关重要的作用。在植物中，生长素作为最重要的激素之一已经被报道在器官发育过程中调控细胞的分裂模式，然而细胞分裂模式如何在时空上被生长素精确调控仍然知之甚少。福建农林大学海峡联合研究院徐通达课题组报道了非经典的植物生长素信号通过类受体蛋白激酶 Transmembrane kinases (TMKs) 调控侧根发育过程中细胞分裂模式的分子机制。研究发现拟南芥 TMK 家族中的两个成员 TMK1 和 TMK4 介导
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