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- 文献和实验
- 技术资料
- 相关疾病：
正常
- ATCC Number：
CRL-1836™
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 生长状态：
贴壁生长
- 细胞类型：
成纤维细胞
- 器官来源：
皮肤
- 库存：
大量
- 运输方式：
冻存运输
- 年限：
66 years
- 细胞形态：
成纤维样
| Designations: | CCD-944Sk | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
||
| Source: | Organ: skin Disease: normal Cell Type: fibroblast |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 12 D5S818: 10,11 D7S820: 7,10 THO1: 9,9.3 TPOX: 8 vWA: 16,18 |
||
| Age: | 66 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The line was established from skin taken from the abdomen. Cells from the current passage (2) have experienced about 14 population doublings beyond the biopsy material Subculture every 6 to 8 days. |
||
| Propagation: | ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate; 90%; fetal bovine serum, 10% |
||
| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 3 to 4 days Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. |
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文献和实验sapiens (human) LS 180 CL-188 Homo sapiens (human) LS 174T CRL-10624 Homo sapiens (human) CO 88BV59-1 CRL-11538 Homo sapiens (human) Co88BV59H21-2 CRL-11539 Homo sapiens (human) Co88BV59H21-2V67-66 CRL-1459 Homo sapiens (human) CCD-18
乳腺管癌细胞 400元 BxPC-3 TCHu 12 人 原位胰腺腺癌细胞 450元 C127 TCM26 小鼠 乳腺肿瘤细胞 400元 C6 TCR 1 大鼠 胶质瘤细胞 1000元 Caco-2 TCHU 66 人 结肠腺癌细胞 400元 Calu-3 TCHu 67 人 肺腺癌 (胸膜渗出液) 400元 CBRH-7919 TCR 2 大鼠 肝癌细胞 400元 CCD-1095Sk GNHu15 人 乳腺浸润性导管癌旁皮肤
胚肺细胞 293 Cells, low passage 人胚肾细胞 293T 人胚肾细胞 AAV-293 胚肾细胞 hFOB 1.19 SV40 转染成骨细胞 CCD-1095Sk 皮肤细胞 Hs 578Bst 乳腺细胞 NK-92 人恶性非霍奇金淋巴瘤患者的自然杀伤细胞, NK-92MI 人恶性非霍奇金淋巴瘤患者的自然
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