MabI内切酶报价

特别提示：包括MabI内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：MabI内切酶
英文名称：MabI
产品货号：GS2120
产品规格：100U

储存条件：冷冻（-20℃）

使用说明

To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	A↑CCWGGT
来源	Microbacterium arborescens SE
Assayed on	Lambda DNA
Optimal buffer	W?(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.)+?BSA
Optimal temperature	37℃
Storage conditions	10mMTris-HCl(pH 7.5);100 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA,50%glycerol;Store at -20℃.
Ligations	After 5-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a.of enzyme for 16hoursat 37℃.
Methlation effect	Not blocked by overlapping Dcm methylation(CmCWGG)ACCWGGT.
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends	CsiI,SexAI

我公司销售的MabI内切酶报价，质量上佳，价格普惠，欢迎广大新老客户踊跃订购。