HspAI内切酶北京品牌

特别提示：包括HspAI内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：HspAI内切酶
英文名称：HspAI
产品货号：GS2096
产品规格：500U

储存条件：冷冻（-20℃）

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	G↑CGC
来源	Isolated from an E.coli strain that carries the cloned HspA I gene from Haemophilus species A1
Assayed on	Lambda DNA
Optimal buffer	Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)
Optimal temperature	37℃
Storage conditions	10 mM Tris-HCl(pH 7.5);50 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.Store at -20℃.
Ligations	After 20-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃.
Methlation effect	Blocked by CG methylation 5-G(5mC)GC-3/3-CG(5mC)G-5.|Not blocked by methylation 5-GCG(5mC)-3/3-CGCG-5.|Cut hemimethylated site:5- G(5mC)GC-3/3-CGCG-5.
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible Ends	AspLEI?^,BstHHI^,CfoI^,GlaI^,HhaI^,R9529,HinP1I

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