- ￥1350 - 3000
- Proteintech
- 66670-1-Ig
- 2025年10月13日
- WB, IHC, ELISA
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human IRF3 protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, IHC, ELISA
- 靶点：
IRF3
- 抗体英文名：
IRF3 Monoclonal antibody
- 抗体名：
IRF3 Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells, THP-1 cells, MOLT-4 cells, HepG2 cells, Jurkat cells, Daudi cells, Karpas-422 cells, Ramos cells |
| Positive IHC detected in | human spleen tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66670-1-Ig targets IRF3 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag27223 Product name: Recombinant human IRF3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-452 aa of BC009395 Sequence: MGTPKPRILPWLVSQLDLGQLEGVAWVNKSRTRFRIPWKHGLRQDAQQEDFGIFQAWAEATGAYVPGRDKPDLPTWKRNFRSALNRKEGLRLAEDRSKDPHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSLAVAPEPCPQPLRSPSLDNPTPFPNLGPSENPLKRLLVPGEEWEFEVTAFYRGRQVFQQTISCPEGLRLVGSEVGDRTLPGWPVTLPDPGMSLTDRGVMSYVRHVLSCLGGGLALWRAGQWLWAQRLGHCHTYWAVSEELLPNSGHGPDGEVPKDKEGGVFDLGPFIVGSWAPRSDYLHGRKRTLTTLCPLVLCGGVMAPGPAVDQEARDGQGCAHVPQGLGRNGPGRGCLLPGEYCGPAHFQQPPTLPHLRPVQGLPAGLGGGHGFPGPWGDLSPRSSWCASNPPVPHHLNQ |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interferon regulatory factor 3 |
| 别名 | 1E6G8, interferon regulatory factor 3, IRF 3, IRF-3 |
| 计算分子量 | 47 kDa |
| 观测分子量 | 50-60 kDa |
| GenBank蛋白编号 | BC009395 |
| 基因名称 | IRF3 |
| Gene ID (NCBI) | 3661 |
| RRID | AB_2882024 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q14653 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB,IF | Front Immunol Swine Acute Diarrhea Syndrome Coronavirus Nucleocapsid Protein Antagonizes Interferon-β Production via Blocking the Interaction Between TRAF3 and TBK1.Authors - Zhihai Zhou |
| human | WB | Vet Microbiol DDX56 antagonizes IFN-β production to enhance EMCV replication by inhibiting IRF3 nuclear translocationAuthors - Shujuan Xu |
| human | WB | Heliyon IDI1 inhibits the cGAS-Sting signaling pathway in hepatocellular carcinomaAuthors - Lin Fu |
| human | WB | Chin Herb Med Anemoside B4 inhibits SARS-CoV-2 replication in vitro and in vivoAuthors - Mingyue Xiao |
| human | WB | Cell Rep Nuclear translocation of cGAS orchestrates VEGF-A-mediated angiogenesisAuthors - Juanjuan Luo |
| human | WB | mSphere Mammalian orthoreovirus capsid protein σ3 antagonizes RLR-mediated antiviral responses by degrading MAVSAuthors - Dianyu Li |
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