- ￥5800
- Proteintech
- 84490-2-PBS
- 2025年10月09日
- WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
- 靶点：
EPCAM/CD326
- 抗体英文名：
Mouse EPCAM/CD326 Recombinant monoclonal antibody, PBS Only (Capture)
- 抗体名：
Mouse EPCAM/CD326 Recombinant monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
84490-2-PBS targets EPCAM/CD326 as part of a matched antibody pair:
MP01344-1: 84490-2-PBS capture and 84490-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg1883 Product name: Recombinant mouse EpCAM/TROP1 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 24-266 aa of NM_008532.2 Sequence: QRDCVCDNYKLATSCSLNEYGECQCTSYGTQNTVICSKLASKCLAMKAEMTHSKSGRRIKPEGAIQNNDGLYDPDCDEQGLFKAKQCNGTATCWCVNTAGVRRTDKDTEITCSERVRTYWIIIELKHKERESPYDHQSLQTALQEAFTSRYKLNQKFIKNIMYENNVITIDLMQNSSQKTQDDVDIADVAYYFEKDVKGESLFHSSKSMDLRVNGEPLDLDPGQTLIYYVDEKAPEFSMQGLT |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | epithelial cell adhesion molecule |
| 别名 | 241851E11, CD326, EGP314, Epcam, Ep-CAM |
| 计算分子量 | 35 kDa |
| 观测分子量 | 35 kDa, 40 kDa |
| GenBank蛋白编号 | NM_008532.2 |
| 基因名称 | Epcam |
| Gene ID (NCBI) | 17075 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q99JW5 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)
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文献和实验Chromosome Conformation Capture (3C) (alternate technique)
Conformation Capture) technique generates a population average measurement of juxtaposition frequency between any two genomic loci, thus providing information on their relative proximity in the nucleus (Dekker et al ., 2002). Cells are fixed with formaldehyde
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
Expanding Mouse Ventricular Cardiomyocytes Through GSK‐3 Inhibition
. , Chiravuri, M. , van der Meer, P. , Feinberg, A.W. , Shi, X. , Shao, Y. , Wu, S.M. , Parker, K.K. , and Chien, K.R. 2009. Generation of functional ventricular heart muscle from mouse ventricular progenitor cells. Science 326:426‐429
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