- ￥1350 - 3000
- Proteintech
- 21775-1-AP
- 2025年10月13日
- WB, IHC, IP, ELISA
- Rabbit
- human
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- 文献和实验
- 技术资料
- 免疫原：
Recombinant human IFIH1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IP, ELISA
- 靶点：
IFIH1/MDA5
- 抗体英文名：
IFIH1/MDA5 Polyclonal antibody
- 抗体名：
IFIH1/MDA5 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Jurkat cells, Raji cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21775-1-AP targets IFIH1/MDA5 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, RIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, pig, monkey |
| 免疫原 |
CatNo: Ag16467 Product name: Recombinant human IFIH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-310 aa of BC111750 Sequence: MSNGYSTDENFRYLISCFRARVKMYIQVEPVLDYLTFLPAEVKEQIQRTVATSGNMQAVELLLSTLEKGVWHLGWTREFVEALRRTGSPLAARYMNPELTDLPSPSFENAHDEYLQLLNLLQPTLVDKLLVRDVLDKCMEEELLTIEDRNRIAAAENNGNESGVRELLKRIVQKENWFSAFLNVLRQTGNNELVQELTGSDCSESNAEIENLSQVDGPQVEEQLLSTTVQPNLEKEVWGMENNSSESSFADSSVVSESDTSLAEGSVSCLDESLGHNSNMGSDSGTMGSDSDEENVAARASPEPELQLRP |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interferon induced with helicase C domain 1 |
| 别名 | IFIH1, MDA5, IFIH 1, Helicard, EC:3.6.4.13 |
| 计算分子量 | 1025 aa, 117 kDa |
| 观测分子量 | 117-125 kDa |
| GenBank蛋白编号 | BC111750 |
| 基因名称 | IFIH1 |
| Gene ID (NCBI) | 64135 |
| RRID | AB_10734593 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BYX4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasionAuthors - Wenwen Zheng |
| WB,RIP | Cell Stem Cell ADAR1-Dependent RNA Editing Promotes MET and iPSC Reprogramming by Alleviating ER Stress.Authors - Diana Guallar | |
| human | WB | Nat Commun MLL5 suppresses antiviral innate immune response by facilitating STUB1-mediated RIG-I degradation.Authors - Peipei Zhou |
| human | WB | Genes Dev PMID: 30463905Authors - Rosa M Rubio |
| human | WB | Nat Commun HIV-1 intron-containing RNA expression induces innate immune activation and T cell dysfunction.Authors - Hisashi Akiyama |
| human | WB | EMBO J CircPVT1 promotes ER-positive breast tumorigenesis and drug resistance by targeting ESR1 and MAVSAuthors - Jia Yi
|
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