- ￥1350 - 3000
- Proteintech
- 17617-1-AP
- 2025年10月13日
- WB, IHC, IF-P, IF-Fro, IP, ELISA
- Rabbit
- human, mouse, rat, pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human CD3E protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat, pig
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF-P, IF-Fro, IP, ELISA
- 靶点：
CD3
- 抗体英文名：
CD3 Polyclonal antibody
- 抗体名：
CD3 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Jurkat cells, pig spleen tissue, MOLT-4 cells, mouse thymus tissue, rat thymus tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue, mouse spleen tissue, human lymphoma tissue, human lung cancer tissue, human breast cancer tissue, mouse colon tissue, human pancreas cancer tissue, rat thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue, mouse spleen tissue |
| Positive IF-Fro detected in | mouse colon tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17617-1-AP targets CD3 in WB, IHC, IF-P, IF-Fro, IP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF-P, IF-Fro, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, Cell treatment |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag11797 Product name: Recombinant human CD3E protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-207 aa of BC049847 Sequence: MQSGTHWRVLGLCLLSVGVWGQDGNEEMGGITQTPYKVSISGTTVILTCPQYPGSEILWQHNDKNIGGDEDDKNIGSDEDHLSLKEFSELEQSGYYVCYPRGSKPEDANFYLYLRARVCENCMEMDVMSVATIVIVDICITGGLLLLVYYWSKNRKAKAKPVTRGAGAGGRQRGQNKERPPPVPNPDYEPIRKGQRDLYSGLNQRRI |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CD3e molecule, epsilon (CD3-TCR complex) |
| 别名 | CD3E, CD3 epsilon, CD3-epsilon, T3E, T-cell surface antigen T3/Leu-4 epsilon chain |
| 计算分子量 | 207 aa, 23 kDa |
| 观测分子量 | 23-25 kDa |
| GenBank蛋白编号 | BC049847 |
| 基因名称 | CD3 |
| Gene ID (NCBI) | 916 |
| ENSEMBL Gene ID | ENSG00000198851 |
| RRID | AB_1939430 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07766 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a polyclonal antibody directed against the epsilon chain of human CD3 molecule.
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文献和实验| Species | Application | Title |
|---|---|---|
| pig | IF | Nature Injectable ultrasonic sensor for wireless monitoring of intracranial signalsAuthors - Hanchuan Tang |
| human | IHC | Blood C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapseAuthors - Li-Xue Yang |
| human | IHC | Nat Commun Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56.Authors - Fan Zou |
| rat | IHC | J Adv Res Zearalenone-14-glucoside specifically promotes dysplasia of Gut-Associated Lymphoid Tissue: A natural product for constructing intestinal nodular lymphatic hyperplasia modelAuthors - Haonan Ruan |
| human | IHC | Cancer Res M6A Demethylase ALKBH5 Regulates PD-L1 Expression and Tumor Immunoenvironment in Intrahepatic Cholangiocarcinoma.Authors - Xinyao Qiu |
Acta Biomater Simultaneous targeting of TGF-β1/PD-L1 via a hydrogel-nanoparticle system to remodel the ECM and immune microenvironment for limiting adhesion formationAuthors - Qian Qian Yang |
/CD3复合体所特有，它可以不依赖CD3的其它亚单位而存在于NK中，并同FcζγRⅢ（CD16）相连。此外，在TCR触发后，ζ链可以同一胞浆内称为ζ链相关蛋白70（ZAP-70）相结合，AP-70为一种胞浆内具有PTK活性的信号蛋白，含有两个SH-2（src homology region 2,SH-2）结构域以及一个与猪脾中PTK syk相关的激酶结构域，ZAP-70分子中SH-2与ζ链中磷酸化的酪氨酸残基相结合，ζ链的酷氨酸磷酸化是由p59 fyn 或p56 lck
【摘要】目的 构建和表达抗CD3/抗Pgp微型双功能抗体，并测定该微型双功能抗体的生物学活性。方法 采用PCR和overlap PCR方法构建抗CD3/抗CD20微型双功能抗体，并用双脱氧终止法测定DNA序列；采用亲和层析法纯化该产物，并用Western blot和分子排阻层析鉴定纯化产物；采用免疫荧光法、放射免疫分析法和玫瑰花环试验鉴定纯化产物与靶细胞的结合活性。结果 DNA序列测定结果表明：抗CD3/抗Pgp微型双功能抗体已构建成功，表达可溶性产物的产量达2mg/l以上，纯化产物中二
和程度，并且考虑是否有必要进行半定量测定。 《一》以抗原表达模式定位 1．细胞质内弥散性分布，多数免疫组织化学染色为胞质型阳性反应，如细胞角蛋白(Cytokeratin，CK)和波形蛋白(Vimentin)等。 免疫组织化学染色(SP法。AEC显色) 胚胎12天人员端脑背侧脑室区司见波形蛋L(Vimentin)免疫反应阳性细胞：其胞体位于脑室区。一端伸出长突起到达软脑膜 2．细胞核周边胞质内分布，其判别要点是细胞核轮廓被勾画得很清楚，如CD3多克隆抗体的染色
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