- ￥1350 - 3000
- Proteintech
- 17260-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human HLA class I (HLA-B) protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
HLA class I (HLA-B)
- 抗体英文名：
HLA class I (HLA-B) Polyclonal antibody
- 抗体名：
HLA class I (HLA-B) Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, Jurkat cells, MCF-7 cells, HeLa cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human stomach cancer tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human spleen tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17260-1-AP targets HLA class I (HLA-B) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag10947 Product name: Recombinant human HLA class I (HLA-B) protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-362 aa of BC013187 Sequence: MRVTAPRTVLLLLSGALALTETWAGSHSMRYFYTAMSRPGRGEPRFISVGYVDDTQFVRFDSDAASPREEPRAPWIEQEGPEYWDRNTQICKTNTQTYRESLRNLRGYYNQSEAGSHTLQRMYGCDVGPDGRLLRGHDQYAYDGKDYIALNEDLSSWTAADTAAQITQRKWEAAREAEQLRAYLEGLCVEWLRRYLENGKETLQRADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEPSSQSTIPIVGIVAGLAVLAVVVIGAVVATVMCRRKSSGGKGGSYSQAASSDSAQGSDVSLTA |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | major histocompatibility complex, class I, B |
| 别名 | HLA-B, MHC class I antigen, Human leukocyte antigen B, HLA class I histocompatibility antigen, B alpha chain, HLA B |
| 计算分子量 | 362 aa, 40 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC013187 |
| 基因名称 | HLA class I (HLA-B) |
| Gene ID (NCBI) | 3106 |
| RRID | AB_1939610 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P01889 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-B can also react with HLA-A and HLA-C. (PMID: 667938; 3375250)
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- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| human | IHC | Gut Single-cell RNA sequencing reveals intrahepatic and peripheral immune characteristics related to disease phases in HBV-infected patients.Authors - Chao Zhang |
| human | IF | Elife Cell-surface tethered promiscuous biotinylators enable comparative small-scale surface proteomic analysis of human extracellular vesicles and cells.Authors - Lisa L Kirkemo |
| human | WB | ACS Chem Biol Engineering Antibodies Targeting p16 MHC-Peptide Complexes.Authors - Nicholas J Rettko |
| human | WB | Invest Ophthalmol Vis Sci Response of Human Retinal Microvascular Endothelial Cells to Influenza A (H1N1) Infection and the Underlying Molecular MechanismAuthors - Shuo Yang |
| human | WB | Cell Death Dis Exosomal annexin A6 induces gemcitabine resistance by inhibiting ubiquitination and degradation of EGFR in triple-negative breast cancer.Authors - Ting Li |
| human | WB | bioRxiv Secreted HLA Fc-Fusion Profiles Immunopeptidome in Hypoxic PDAC and Cellular SenescenceAuthors - Nicholas J Rettko |
Generation of HLA Class I Transfected Target Cell Lines
Normal human lymphoblastoid cell lines (LCLs) express six classical class I HLA genes from three gene loci, HLA-A, HLA-B, and HLA-C, which are located within the major histocompatibility complex (MHC). Because of the extensive allelic
强直性脊柱炎（AS）是一种以脊柱为主要病变的慢性自身免疫性疾病,该病的发展会造成不同程度的骨骼、肌肉、眼、肺病变。虽然这是一种很古老的疾病，几千年前古埃及人的骨骼中就发现有强直性脊柱炎的证据，在2000年以前希腊名医希波克拉底就对此病进行了描述，但其病因和致病机理却至今尚未阐明。但在众多病因研究结果中，遗传基因HLA-B*27是最受公认的诱因，它与AS的相关性早在40年前就被认识到了。因此检测该基因的携带情况对AS的诊断具有很大帮助，本文将着重介绍该基因的基因芯片检测技术。
Establishing MHC Class I Peptide Motifs
and to compose a reliable scoring matrix for epitope prediction. As an example, a scoring matrix for the prediction of HLA-B*35-presented T-cell epitopes will be developed by examining the characteristics of 76 naturally presented HLA ligands.
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