- ￥1350 - 3000
- Proteintech
- 13801-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human SERPINE1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
Serpin E1/PAI-1
- 抗体英文名：
Serpin E1/PAI-1 Polyclonal antibody
- 抗体名：
Serpin E1/PAI-1 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HepG2 cells, HuH-7 cells, L02 cells, MG-63 cells, U2OS cells, human placenta tissue |
| Positive IP detected in | HUVEC cells |
| Positive IHC detected in | human placenta tissue, human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13801-1-AP targets Serpin E1/PAI-1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, bovine |
| 免疫原 |
CatNo: Ag4777 Product name: Recombinant human SERPINE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-402 aa of BC010860 Sequence: MQMSPALTCLVLGLALVFGEGSAVHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPKFSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASSSTAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 |
| 别名 | PAI-1, SERPINE1, SERPINE1, PAI-1, PAI, PAI 1 |
| 计算分子量 | 402 aa, 45 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC010860 |
| 基因名称 | PAI-1 |
| Gene ID (NCBI) | 5054 |
| RRID | AB_2186881 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P05121 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. In western blotting, we got two bands 50 kDa and 90-110 kDa, and 90-110 kDa consistent with PAI-1 in complex with its target protease, t-PA (PMID: 21596853).
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Am J Respir Crit Care Med CD38 Mediates Lung Fibrosis by Promoting Alveolar Epithelial Cell AgingAuthors - Huachun Cui |
| human | WB | Cancer Discov Dynamic glycoprotein hyposialylation promotes chemotherapy evasion and metastatic seeding of quiescent circulating tumor cell clusters in breast cancerAuthors - Nurmaa K Dashzeveg |
| human | WB,IHC,CoIP | Circ Res MiR-18a inhibits BMP4 and HIF-1α Normalizing Brain Arteriovenous Malformations.Authors - Nagore I Marín-Ramos |
| human | WB,IHC,IF | Nat Commun A translational program that suppresses metabolism to shield the genome.Authors - Nathan C Balukoff |
| human | WB | Nat Commun ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.Authors - Rokana Taftaf |
| human | WB | Nat Commun A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism.Authors - J J David Ho |
14-3-3 sigma 14-3-3 sigma亚硫酸钠修饰的DNA 14-3-3 zeta c-Fos FosB Fra-1 Fra-2 c-Jun JunB JunD Fibronectin PAI-1 M16006 PAI-1 XM009352 GM-CSF FHIT IL-4 CD2AP real-time RT-PCR CCAAT/enhancer binding protein (C/EBP)：alpha / beta / delta
11种补体调节分子的特性及生物学活性见表5-2 表5-2 补体调节分子的特性及生物学活性 调节分子 分子量（kDa）（分子特征） 血清浓度（μg/ml）或细胞分布 能特异性相互作用的分子 生物学活性 血清蛋白： C1INH 104 200 C1r,C1s 与C1r和C1s共价结合使C1失活； （serpin） 与C1结合阻止其自发激活：抑制激肽释放酶、纤溶酶及凝血因子Ⅻa、Ⅺa C4bp 550（12个SCR） 250 C4b 抑制CP中的C3转化酶形成
by 35S-methionine For more details about procedure and controls see: Kzhyshkowska J et al, Heterogeneous nuclear ribonucleoprotein E1B-AP5 is methylated in its Arg-Gly-Gly (RGG) box and interacts with human arginine methyltransferase HRMT1L1.
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