- ￥1350 - 3000
- Proteintech
- 12063-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human KRAS protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- 靶点：
KRAS
- 抗体英文名：
KRAS Polyclonal antibody
- 抗体名：
KRAS Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, HEK-293 cells, human brain tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, rat liver tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells, HGC-27 cells, MKN-45 cells, mouse brain tissue |
| Positive IHC detected in | human lung cancer tissue, human kidney tissue, human pancreas cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12063-1-AP targets KRAS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken, zebrafish |
| 免疫原 |
CatNo: Ag2700 Product name: Recombinant human KRAS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-188 aa of BC013572 Sequence: MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
| 别名 | pan RAS, C K RAS, c Ki ras, c-Ki-ras, c-K-ras |
| 计算分子量 | 188 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC013572 |
| 基因名称 | KRAS |
| Gene ID (NCBI) | 3845 |
| RRID | AB_878040 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P01116 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Nat Biotechnol Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors.Authors - Christopher W Davies |
| human | WB | Cancer Discov LZTR1 mutation mediates oncogenesis through stabilization of EGFR and AXLAuthors - Aram Ko |
| mouse | WB,IHC | Cell Res In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.Authors - Zheng Fu |
| mouse | WB | Cancer Discov Systematic functional interrogation of rare cancer variants identifies oncogenic alleles.Authors - Eejung Kim |
| human | WB | Mol Cell The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.Authors - Linyu Sun |
| human | WB | Mol Cell Regulation of GTPase function by autophosphorylation.Authors - Christian W Johnson |
C. David/赵玉沛院士/吴文铭揭示「癌王发迹史」，为早期诊断与治疗提供潜在的靶点和思路
比较发现，PDLP 中大量高激活基因的附近，其染色质也变得更加开放。这种开放的染色质区域也会随着炎症的消失而下调，但是会被 Kras 突变基因所维持与增强（图一，e）。通过与 H3K27ac，H3K4me ChIP-seq 数据进行比较发现，这些被 Kras 突变体所维持的染色质开放区域更多地富集在基因组的 enhancer 区域。Kras 调控的转录网络促进癌症的发生发展Kras 作为上游的信号因子，调控非常多的信号通路来促进基因的转录。作者进行了转录因子的 motif search，发现 AP
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验，请根据说明书列出已验证的种属来选择适合你实验的抗体。 （4）样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体，待测样本蛋白的结构域和样本在提取和处理过程中是否会变性，蛋白空间构象的改变，会影响抗体的免疫亲和反应。 （5）单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免
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