- ￥980 - 6000
- Proteintech
- 10866-1-AP
- 2025年10月13日
- WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Porin protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA
- 靶点：
VDAC1/2
- 抗体英文名：
VDAC1/2 Polyclonal antibody
- 抗体名：
VDAC1/2 Polyclonal antibody
- 规格：
100ul/500ul/1ml
| 规格： | 100ul | 产品价格： | ￥980.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥4000.0 |
| 规格： | 1ml | 产品价格： | ￥6000.0 |
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse kidney tissue, 4T1 cells, Jurkat cells, C6 cells, rat kidney tissue, MCF-7 cells, MDA-MB-453s cells |
| Positive IHC detected in | human colon tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue, human liver cancer tissue |
| Positive IF-Fro detected in | mouse liver tissue |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10866-1-AP targets VDAC1/2 in WB, IHC, IF-P, IF-Fro, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, rabbit, monkey, zebrafish, pieris rapae |
| 免疫原 |
CatNo: Ag1144 Product name: Source: e coli.-derived, PGEX-4T Tag: GST Domain: of Sequence: |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | voltage-dependent anion channel 1 |
| 别名 | Porin, VDAC1, VDAC1/Porin, hVDAC1, Plasmalemmal porin |
| 计算分子量 | 31 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | BC008482 |
| 基因名称 | Porin |
| Gene ID (NCBI) | 7416 |
| RRID | AB_2257153 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P21796 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This polyclonal antibody raised against full-length human VDAC1 protein can cross react with VDAC2.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagyAuthors - Yang Meng |
| mouse | WB | Nat Cell Biol AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.Authors - Meng Shi |
| human | WB | Cell Rep Med Urolithin A improves muscle strength, exercise performance, and biomarkers of mitochondrial health in a randomized trial in middle-aged adults.Authors - Anurag Singh |
| human | WB | Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.Authors - Pengli Zheng |
| human | WB | Acta Pharm Sin B Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.Authors - Xin Sun |
| mouse | WB | Circ Res NAD+ Repletion Reverses Heart Failure With Preserved Ejection Fraction.Authors - Dan Tong |
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
Microarray Approaches for Analysis of Cell Cycle Regulatory Genes
Cell cycle phase Mammalian Yeast G0/G1 AP-1 family (FOS, JUN)
同时，Apaf―1可以激活原形的caspase―9，形成细胞色素C／Apaf―1／caspase―9复合体，即凋亡复合体(apoptosome)。该复合体水解并激活下游的caspase通路如caspase―3等。 凋亡复合体的形成是凋亡信号启动的关键步骤。目前发现激活的癌基因可以辅助凋亡复合体的形成，比如腺病毒EIA可以上调Apaf―1和caspase―9，E2F1可以上调Apaf―1的表达。Apaf―1的释放可促进凋亡复合体的形成，对凋亡的启动起促进作用。目前的研究认为，细胞
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