- ￥980 - 6000
- Proteintech
- 10494-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- Rabbit
- human, mouse, rat
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
AJE 润色服务限时 8 折起，权威机构信任 15+ 年Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human GAPDH protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- 靶点：
GAPDH
- 抗体英文名：
GAPDH Polyclonal antibody
- 抗体名：
GAPDH Polyclonal antibody
- 规格：
100ul/500ul/1ml
| 规格： | 100ul | 产品价格： | ￥980.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥4000.0 |
| 规格： | 1ml | 产品价格： | ￥6000.0 |
经过测试的应用
| Positive WB detected in | human placenta tissue, HepG2 cells, HEK-293 cells, HeLa cells, Raji cells, K-562 cells, mouse heart tissue, PC-13 cells, arabidopsis whole plant tissue, corn whole plant tissue, mouse brain tissue, mouse skin tissue, Jurkat cells, NIH/3T3 cells, C6 cells, rat brain tissue, RAW 264.7 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:40000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10494-1-AP targets GAPDH in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat, chicken, goat, yeast, leech, treeshrew, cavia porcellus (domestic guinea pig), arabidopsis, zebra finches |
| 免疫原 |
CatNo: Ag0766 Product name: Recombinant human GAPDH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-335 aa of BC004109 Sequence: MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glyceraldehyde-3-phosphate dehydrogenase |
| 别名 | GAPD, Glyceraldehyde-3-phosphate dehydrogenase |
| 计算分子量 | 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC004109 |
| 基因名称 | GAPDH |
| Gene ID (NCBI) | 2597 |
| RRID | AB_2263076 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P04406 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
询价记录
暂无询价记录
文献和实验GAPDH抗体|GAPDH抗体大全-艾美捷指导您购买合适的GAPDH抗体
书 E021010 EarthOx Anti-GAPDH Monoclonal Antibody 小鼠 WB, IF 效价极高，检测种属多 点击 10494-1-AP ProteinTech
Blotting实验，至内参量一致为止；若内参一致，即可进行不同样品间目的蛋白表达变化分析。常用的蛋白质内参有GAPDH和细胞骨架蛋白beta-actin或beta-tubulin。最近的国外文献报道中使用GAPDH内参的较多。：GAPDH（甘油醛-3-磷酸脱氢酶）是参与糖酵解的一种关键酶，由4个30－40kDa的亚基组成，分子量146kDa。GAPDH基因几乎在所有组织中都高水平表达，广泛用作Western blot蛋白质标准化的内参。GAPDH检测。Western Blotting(检测条带大约在36
可以通过的三维网状结构（即聚丙烯酰胺凝胶）。配胶时会用到催化剂（AP）和加速剂（如 TEMED）、丙烯酰胺（原料）、Tris-HCl 等。AP（即过硫酸铵）能提供驱动丙烯酰胺和亚甲丙烯酰胺聚合所必需的自由基。由于过硫酸铵会缓慢分解，故应隔周新鲜配制，且置于 4 度保存。TEMED (即 N,N,N』,N』- 四甲基乙二胺) 通过催化 AP 形成自由基而加速丙烯酰胺和 N,N』- 亚甲丙烯酰胺的聚合。在加速剂和催化剂的作用下，丙烯酰胺会形成长链聚合物。我们可通过改变丙烯酰胺和双丙烯酰胺的浓度来控制凝胶
技术资料暂无技术资料 索取技术资料
