- ￥1350 - 3000
- Proteintech
- 10143-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human KRT14 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- 靶点：
Cytokeratin 14
- 抗体英文名：
Cytokeratin 14 Polyclonal antibody
- 抗体名：
Cytokeratin 14 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A431 cells, rat skin tissue |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human lung cancer tissue, human cervical cancer tissue, human breast cancer tissue, human skin tissue, human skin cancer tissue, human oesophagus cancer tissue, mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HaCaT cells, HeLa cells, HepG2 cells, mouse olfactory epithelium tissue |
| Positive FC (Intra) detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10143-1-AP targets Cytokeratin 14 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag0188 Product name: Recombinant human KRT14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 262-472 aa of BC002690 Sequence: GQVGGDVNVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEEWFFTKTEELNREVATNSELVQSGKSEISELRRTMQNLEIELQSQLSMKASLENSLEETKGRYCMQLAQIQEMIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLSSSQFSSGSQSSRDVTSSSRQIRTKVMDVHDGKVVSTHEQVLRTKN |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | keratin 14 |
| 别名 | KRT14, keratin 14, Cytokeratin-14, Cytokeratin,Keratin, CK-14 |
| 计算分子量 | 472 aa, 52 kDa |
| 观测分子量 | 47-50 kDa |
| GenBank蛋白编号 | BC002690 |
| 基因名称 | Cytokeratin 14 |
| Gene ID (NCBI) | 3861 |
| RRID | AB_2134831 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P02533 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | IHC | Cell Stem Cell Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.Authors - Brian Lin |
| mouse | IF | Adv Sci (Weinh) Photothermal-Responsive Soluble Microneedle Patches for Meibomian Gland Dysfunction TherapyAuthors - Fei Yu |
| mouse | IF | Nat Commun Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica.Authors - Yue Hong |
| human | IF | Nat Commun Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure.Authors - Vlasta Lungova |
| mouse | IF | Biomaterials MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.Authors - Tian Wang |
| human | WB | Oncogene EB-virus latent membrane protein 1 potentiates the stemness of nasopharyngeal carcinoma via preferential activation of PI3K/AKT pathway by a positive feedback loop.Authors - C-F Yang |
AP Assay and FGF Binding Mike Naski COS-7 cells were transfected by a standard DEAE-dextran method with 4 mg of plasmid/10 6 cells. Conditioned medium was harvested 3, 6 and 9 days after transfection and used for AP enzyme
Stem Cell Rep：高绍荣团队揭示 Mettl14 以不依赖于 m⁶A 的方式驱动衰老相关分泌表型促进体细胞重编程
的方式调控 SASP 基因表达水平 6。因此，研究者推测，在重编程晚期，Mettl14 上调的这些基因为 SASP 基因。进一步研究发现，在重编程晚期，这些 SASP 基因的表达水平显著升高，但是，在建系的 iPSC 中几乎不表达。 为了进一步研究 SASP 与重编程的关系，研究者进行了细胞上清共培养实验及 SASP 因子共培养实验 (图 2)。结果表明，重编程过程的细胞上清或 SASP 因子（如 IL6）可以促进重编程后期的细胞转变为多能干细胞。同时，对细胞进行碱性磷酸酶 AP 染色和 β
流速为14～16滴/min。分部收集器收集，紫外监测，或人工收集，以紫外分光光度计分别测定每管280nm OD值，描绘洗脱液峰。 6. 浓缩：将洗脱液的280nm OD上峰段与下峰段各管分别合并，装入透析袋，以PEG浓缩至所需体积。 三、Tris-PO4 离子交换层析法 该法在上述方法的基础上稍加改良，即通过梯度洗脱，可用于血清中IgG和IgM的提取。 材料和试剂 1. DE32或DE52纤维素。 2. 待纯化标本：杂交瘤腹水或培养上清，免疫血清或饱和硫酸铵粗提品。
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