- ￥1350 - 3000
- Proteintech
- 10749-1-AP
- 2025年10月13日
- WB, IF/ICC, IP, ELISA
- Rabbit
- human, mouse, rat
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- 文献和实验
- 技术资料
- 免疫原：
Recombinant human RHOA protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IF/ICC, IP, ELISA
- 靶点：
RHOA
- 抗体英文名：
RHOA Polyclonal antibody
- 抗体名：
RHOA Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, rat brain tissue, PC-12 cells, HL-60 cells, mouse brain tissue, HUVEC cells, NIH/3T3 cells, fetal human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10749-1-AP targets RHOA in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, canine |
| 免疫原 |
CatNo: Ag1141 Product name: Recombinant human RHOA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-193 aa of BC005976 Sequence: MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ras homolog gene family, member A |
| 别名 | ARH12, ARHA, RHO12, EC:3.6.5.2, h12 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC005976 |
| 基因名称 | RHOA |
| Gene ID (NCBI) | 387 |
| RRID | AB_2285104 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P61586 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
What is the function of RhoA?
Ras homolog gene family, member A (RhoA) is a small GTPase that is involved in cytoskeleton organization.1 It is important in actomyosin contractility, as when RhoA binds to GTP it initiates a cascade of events mediating stress fiber or contractile ring formation, where actin bundles crosslink with other proteins. It is also associated with actin polymerization, making RhoA important in membrane ruffling and cell motility.2 The role of RhoA is essential in cell migration and adhesion.
What is the cellular localization of RhoA?
In motile cells, a leading edge stretches ahead, stabilizes, the cytoskeleton is reorganized, and the tail of the cell retracts. The role of RhoA in contracting actomyosin has linked RhoA with the tail retraction in this process, but studies into the spatiotemporal kinetics of GTPases have revealed that RhoA is also located at the leading edge of migrating cells.3 This highlights the dual roles of this protein in actin contractility and polymerization.
What is the role of RhoA in disease?
Though it is not an oncogene, the role of RhoA in adhesion and migration has implicated this protein in cancer biology. Expression of RhoA has been found to be higher in malignant tumors compared to benign tumors or in non-tumor tissue, and more invasive tumors have been found to overexpress RhoA.4 This protein is also implicated in neurodegenerative diseases and aging due to its role in axon guidance, with abnormal localization of RhoA associated with Alzheimer's and Parkinson's pathology.5,6
1. Ridley, A. J. & Hall, A. The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell 70, 389-399 (1992).
2. 21. Kurokawa, K. & Matsuda, M. Localized RhoA Activation as a Requirement for the Induction of Membrane Ruffling. Mol. Biol. Cell 16, 4294-4303 (2005).
3. 22. Struckhoff, A. P., Rana, M. K. & Worthylake, R. A. RhoA can lead the way in tumor cell invasion and metastasis. Front. Biosci. (Landmark Ed. 16, 1915-26 (2011).
4. 23. Mcglasson, S. et al. Rare variants of the 3'-5' DNA exonuclease in early onset TREX1 small vessel stroke [version 1; referees: awaiting peer review]. doi:10.12688/wellcomeopenres.12631.1
5. 24. Huesa, G. et al. Altered Distribution of RhoA in Alzheimer's Disease and AβPP Overexpressing Mice. J. Alzheimer's Dis. 19, 37-56 (2010).
6. 25. Hynds, D. L. Subcellular localization of Rho GTPases: implications for axon regeneration. Neural Regen. Res. 10, 1032-3 (2015).
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文献和实验因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验，请根据说明书列出已验证的种属来选择适合你实验的抗体。 （4）样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体，待测样本蛋白的结构域和样本在提取和处理过程中是否会变性，蛋白空间构象的改变，会影响抗体的免疫亲和反应。 （5）单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免
发生，最好在特异性抗体处理切片之前，选择与二抗种属相同的非免疫血清封闭电荷，阻止一抗与之结合，抑制非特异性背景着色。常用方法是用2%～10%羊血清或2%～5%牛血清白蛋白在室温下作用10~30分钟。但应注意此种结合不牢固，所以最好不要冲洗，倾去余液直接加一抗。SABC或SP免疫组织化学试剂盒中常配有非免疫血清。 7．第一抗体孵育 滴加特异性一抗于切片上，4℃孵育24～48小时，或室温孵育过夜，也可在37~C孵育1～2小时。之后，用PBS冲洗3次，每次5分钟。一抗分为多克隆抗体和单克
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