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- 可靠的定量检测遗传学或表观遗传学DNA修饰
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文献和实验Boric Acid 0.02M Disodium EDTA ) (Advanced Biotechnologies)Acrylease (Stratagene)Glass cleaner (e.g., Windex, Glass Plus)95% ethanol2. EquipmentThermal cycler (MJ Research)Sequencing gel electrophoresis apparatus (Gibco BRL)High voltage power supplyGel
This method is used to detect genomic DNA deletions in tumor cells. For a more detailed discussion of applying this approach to microdissected samples, see Allelic Loss Studies in Prostate MP at NCI . 1. Reagents DNA sample
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
-anchored PCR (21),single-specific-primer PCR (22), rapid amplification of cDNA ends(23, 24), and rapid amplification of genomic DNA ends (25). ThisPCR begins with the digestion of genomic DNA with a restrictionenzyme, followed by ligation of a double
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