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- 电转化感受态细胞
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- 2025年11月16日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Endura™ ElectroCompetent Cells
- 供应商：
Lucigen
Lucigen感受态细胞选择指南：
产品货号列表
| 货号 | 产品名称 | 规格 |
| 60051-1 | E. cloni® 10G ELITE Electrocompetent Cells | 12 rxns (SOLOs) |
| 60051-2 | E. cloni® 10G ELITE Electrocompetent Cells | 24 rxns (SOLOs) |
| 60052-1 | E. cloni® 10G ELITE Electrocompetent Cells | 12 rxns (DUOs) |
| 60052-2 | E. cloni® 10G ELITE Electrocompetent Cells | 24 rxns (DUOs) |
| 60052-3 | E. cloni® 10G ELITE Electrocompetent Cells | 24 rxns (SixPacks) |
| 60052-4 | E. cloni® 10G ELITE Electrocompetent Cells | 48 rxns (SixPacks) |
| 60061-1 | E. cloni® 10GF' ELITE Electrocompetent Cells | 12 rxns (DUOs) |
| 60061-2 | E. cloni® 10GF' ELITE Electrocompetent Cells | 24 rxns (DUOs) |
| 60080-1 | E. cloni® 10G SUPREME Electrocompetent Cells | 12 rxns (DUOs) |
| 60080-2 | E. cloni® 10G SUPREME Electrocompetent Cells | 24 rxns (DUOs) |
| 60081-1 | E. cloni® 10G SUPREME Electrocompetent Cells | 12 rxns (SOLOs) |
| 60081-2 | E. cloni® 10G SUPREME Electrocompetent Cells | 24 rxns (SOLOs) |
| 60096-1 | E. cloni® 10G Chemically Competent Cells | 1 plate |
| 60096-4 | E. cloni® 10G Chemically Competent Cells | 4 plates |
| 60106-1 | E. cloni® 10G Chemically Competent Cells | 12 rxns (SOLOs) |
| 60106-2 | E. cloni® 10G Chemically Competent Cells | 24 rxns (SOLOs) |
| 60106-3 | E. cloni® 10G Chemically Competent Cells | 48 rxns (SOLOs) |
| 60107-1 | E. cloni® 10G Chemically Competent Cells | 12 rxns (DUOs) |
| 60107-2 | E. cloni® 10G Chemically Competent Cells | 24 rxns (DUOs) |
| 60107-3 | E. cloni® 10G Chemically Competent Cells | 48 rxns (DUOs) |
| 60107-4 | E. cloni® 10G Chemically Competent Cells | 96 rxns (DUOs) |
| 60108-1 | E. cloni® 10G Chem Comp Cells (Subcloning Grade) | 48 rxns |
| 60108-2 | E. cloni® 10G Chem Comp Cells (Subcloning Grade) | 96 rxns |
| 60110-1 | HI-Control™ 10G Chemically Competent Cells | 12 rxns (SOLOs) |
| 60117-1 | E. cloni® 10G CLASSIC Electrocomp Cells | 24 rxns (SixPacks) |
| 60117-2 | E. cloni® 10G CLASSIC ElectrocompCells | 48 rxns (SixPacks) |
| 60210-1 | BAC-Optimized Replicator™ v2.0 Electrocomp Cell | 12 rxns (DUOs) |
| 60210-2 | BAC-Optimized Replicator™ v2.0 Electrocomp Cell | 24 rxns (DUOs) |
| 60215-2 | E. cloni® 10G BAC-Optimized Electrocomp Cells | 25 rxns (FIVERs) |
| 60224-1 | BigEasy®-TSA™ Electrocompetent Cells | 6 rxns (SOLOs) |
| 60224-2 | BigEasy®-TSA™ Electrocompetent Cells | 12 rxns (SOLOs) |
| 60224-3 | BigEasy®-TSA™ Electrocompetent Cells | 24 rxns (SOLOs) |
| 60240-1 | Endura™ Chemically Competent Cells | 12 rxns (DUOs) |
| 60240-2 | Endura™ Chemically Competent Cells | 24 rxns (DUOs) |
| 60241-1 | Endura™ Chemically Competent Cells | 12 rxns (SOLOs) |
| 60241-2 | Endura™ Chemically Competent Cells | 24 rxns (SOLOs) |
| 60242-1 | Endura™ Electrocompetent Cells | 12 rxns (DUOs) |
| 60242-2 | Endura™ Electrocompetent Cells | 24 rxns (DUOs) |
| 60300-1 | E. cloni® EXPRESS BL21(DE3) Electrocompetent Cells | 12 rxns |
| 60300-2 | E. cloni® EXPRESS BL21(DE3) Electrocompetent Cells | 24 rxns |
| 60341-1 | OverExpress™ C41(DE3) Electrocompetent Cells | 12 rxns (SOLOs) |
| 60341-2 | OverExpress™ C41(DE3) Electrocompetent Cells | 24 rxns (SOLOs) |
| 60345-1 | OverExpress™ C43(DE3) Electrocompetent Cells | 12 rxns (SOLOs) |
| 60345-2 | OverExpress™ C43(DE3) Electrocompetent Cells | 24 rxns (SOLOs) |
| 88891-8 | ZhongBei™ LinGe(Bio）Biotechnology Cells | 86 rxns(SOLOs) |
| 60401-1 | E. cloni® EXPRESS BL21(DE3)Chemically Competent Cells | 12 rxns |
| 60401-2 | E. cloni® EXPRESS BL21(DE3)Chemically Competent Cells | 24 rxns |
| 60401-3 | E. cloni® EXPRESS BL21(DE3)Chemically Competent Cells | 48 rxns |
| 60435-1 | HI-Control® BL21(DE3) Chem Comp Cells | 12 rxns (SOLOs) |
| 60442-1 | OverExpress™ C41(DE3) Chem Comp Cells | 12 rxns (SOLOs) |
| 60442-2 | OverExpress™ C41(DE3) Chem Comp Cells | 24 rxns (SOLOs) |
| 60444-1 | OverExpress™ C41(DE3) pLysS Chem Comp Cells | 12 rxns (SOLOs) |
| 60444-2 | OverExpress™ C41(DE3) pLysS Chem Comp Cells | 24 rxns (SOLOs) |
| 60446-1 | OverExpress™ C43(DE3) Chem Comp Cells | 12 rxns (SOLOs) |
| 60446-2 | OverExpress™ C43(DE3) Chem Comp Cells | 24 rxns (SOLOs) |
| 60448-1 | OverExpress™ C43(DE3) pLysS Chem Comp Cells | 12 rxns (SOLOs) |
| 60448-2 | OverExpress™ C43(DE3) pLysS Chem Comp Cells | 24 rxns (SOLOs) |
| 60452-1 | OverExpress™ ChemComboPack | 12 rxns (SOLOs) |
| 60502-1 | TG1 Electrocompetent Cells | 12 rxns (DUOs) |
| 60502-2 | TG1 Electrocompetent Cells | 24 rxns (DUOs) |
| 60512-1 | SS320 (MC1061 F') Electrocompetent Cells | 12 rxns (DUOs) |
| 60512-2 | SS320 (MC1061 F') Electrocompetent Cells | 24 rxns (DUOs) |
| 60514-1 | MC1061 F- Electrocompetent Cells | 12 rxns (DUOs) |
| 60514-2 | MC1061 F- Electrocompetent Cells | 24 rxns (DUOs) |
| 60522-1 | ER2738 Electrocompetent Cells | 12 rxns (DUOs) |
| 60522-2 | ER2738 Electrocompetent Cells | 24 rxns (DUOs) |
| 60602-1 | E. cloni® 5-alpha Chemically Competent Cells | 12 rxns (DUOs) |
| 60602-2 | E. cloni® 5-alpha Chemically Competent Cells | 24 rxns (DUOs) |
| 60701-1 | CJ236 Electrocompetent Cells | 12 rxns (DUOs) |
| 60701-2 | CJ236 Electrocompetent Cells | 24 rxns (DUOs) |
| 60810-1 | ClearColi® BL21(DE3) Electrocompetent Cells | 12 rxns (DUOs) |
| 60810-2 | ClearColi® BL21(DE3) Electrocompetent Cells | 24 rxns (DUOs) |
| Blue-white capabl | Phage T1 resistant | End A -/RecA | Restriction-deficient (RMS) | Clone methylated DNA | Inducible plasmid copy number | F’episome | Antibiotic resistance | Electrocompetent:efficiency | Chemically competent:efficiency | |
| 常规克隆及文库构建 | ||||||||||
| E. cloni® 10G (Classic) | √ | √ | √ | √ | √ | Ø | Ø | Str | ≥5 x109 | 106-109 |
| E. cloni 10G (Elite) | √ | √ | √ | √ | √ | Ø | Ø | Str | ≥2 x1010 | - |
| E. cloni 10G (Supreme) | √ | √ | √ | √ | √ | Ø | Ø | Str | ≥4 x1010 | - |
| E.cloni 10G F’（Elite) | √ | √ | √ | √ | √ | Ø | √ | Str | ≥2x1010 | - |
| E. cloni 5-alpha | √ | √ | √ | Ø | Ø | Ø | Ø | - | - | ≥1 x108 |
| 不稳定的插入物或有毒基因 | ||||||||||
| Copycutter™ EPI400™ | √ | √ | √ | √ | √ | √ | Ø | Str | ≥1 x1010 | ≥1 x107 |
| 大片段,plasmids和fosmids质粒 | ||||||||||
| TransforMax™ EC100™ | √ | Ø | √ | √ | √ | Ø | Ø | Str | ≥1 x1010 | ≥5 x108 |
| TransforMax EPI300™ | √ | Ø | √ | √ | √ | a | Ø | Str | ≥1 x1010 | ≥5 x108 |
| TransforMax EPI300™-T1R | √ | √ | √ | √ | √ | a | Ø | Str | ≥1 x1010 | |
| BAC克隆 | ||||||||||
| BAC-Optimised Replicator™ v2.0 | √ | Ø | √ | √ | √ | a | Ø | Amp, Str | ≥1 x1010 | - |
| E. cloni BAC-Optimised 10G | √ | Ø | √ | √ | √ | Ø | Ø | Str | ≥1 x1010 | - |
| BigEasy™-TSA | b | √ | √ | √ | √ | c | Ø | Amp, Str | ≥2 x1010 | - |
| 噬菌体展示 | ||||||||||
| TG1 | √ | Ø | Ø | Ø | d | Ø | √ | - | ≥4 x1010 | - |
| ER2738 | √ | √ | Ø | Ø | d | Ø | √ | Tet | ≥2 x1010 | - |
| SS320 (MC1061 F’) | √ | Ø | Ø | Ø | e | Ø | √ | Tet, Str | ≥4 x1010 | - |
| MC1061 F- | Ø | Ø | Ø | Ø | e | Ø | Ø | Str | ≥4 x1010 | - |
| CRISPR sgRNA文库及慢病毒质粒克隆 | ||||||||||
| Endura™ | Ø | Ø | f | Ø | g | Ø | Ø | Str | ≥1 x1010 | |
| Site-directed mutagenesis | ||||||||||
| CJ236 | Ø | Ø | Ø | Ø | Ø | Ø | √ | Cam | ≥1 x109 | - |
| Amp, ampicillin; Cam, chloramphenicol; Str, streptomycin; Tet, tetracycline |
| a: Requires OriV vectors (e.g., CopyRight® and pCCFOS) |
| b: Requires IPTG |
| c: Only with pJAZZ® vectors |
| d: mcrB- only; not suitable for cloning eukaryotic genomic DNA |
| e: mcrA- mcrB-; not suitable for cloning eukaryotic genomic DNA |
| f: RecA13 only |
| g: mcrB- mrr-; not suitable for cloning eukaryotic genomic DNA |
| Primary promoter | Background expression control | Low endotoxin | Antibiotic resistance | Electrocompetent:efficiency | Chemically competent:efficiency | |
| 常规蛋白表达 | ||||||
| E. cloni EXPRESS BL21(DE3) | T7 | Ø | Ø | - | ≥5 x 109 | ≥1 x 107 |
| Hl-Control™ BL21(DE3) | T7 | √ | Ø | Gen | - | ≥1 x 107 |
| Hl-Control™ 10G | Plac,Ptac,Ptrc,T5lac,rhaPBAD | √ | Ø | Gen, Str | - | ≥1 x 109 |
| ClearColi® BL21(DE3) | T7 | Ø | √ | - | ≥1 x 109 | - |
| 带毒性蛋白表达 | ||||||
| OverExpress™ C41(DE3) | T7 | Ø | Ø | - | ≥1 × 109 | ≥1 × 106 |
| OverExpress C41(DE3) pLysS | T7 | √ | Ø | Cam | - | ≥1 × 106 |
| OverExpress C43(DE3) | T7 | Ø | Ø | - | ≥1 × 109 | ≥1 × 106 |
| OverExpress C43(DE3) pLysS | T7 | √ |
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，扩增目标片段 扩增片段与线性化载体、组装预混酶混合反应 转化感受态细胞 · Step 1 · 如果载体上正好有合适插入目标片段的酶切位点，酶切载体使之线性化。为了降低单酶切载体不完全的几率，减少载体自连和提高筛选成功率，强烈建议双酶切——后面一个切点只要距离第一个切点 5 个碱基以上就行（主要是给两个酶留出结合空间）。如果没有合适的酶切位点，根据选定插入位点设计引物，用反向 PCR 来获得线性化载体。在多片段组装克隆的世界里，凡是酶切搞不定的，统统 PCR 引物设计搞定。简单粗暴有效。 ·
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Lucigen™感受态细胞
¥88891086
