相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
无
| |
||||||||
|
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验TOP10 chemically competent cells
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware, and simply rinse it out. Autoclaving glassware filled
间自由转移,因为此时的甲基化模式已经不再为外源性。将DNA导入K-限制菌株[如NEB5-alpha感受态细胞(NEB #C2987)、NEB 5-alpha F´Iq 感受态细胞 (NEB #C2992)或者NEB Turbo 感受态细胞 (NEB #C2984)]之前须肯定DNA为K-修饰模式。 参考文献: (1) Bickle, T. (1993) in Nucleases eds Linn, S.M., Lloyd, R.S. and Roberts, R.J. (CSH, NY) 89
In vitro Mutagenesis with dut ung single stranded DNA
X synthesis Buffer 0.3 microliter Ligase (NEB) 0.3 microliter (3 units) Unit T7 DNA polymerase (NEB) (The above buffer and enzymes can all be mixed just before addition to rxn if desired) Incubate 5 min at 0 deg. Incubate 5 min at room temp.
技术资料暂无技术资料 索取技术资料





