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Recombinant Rat Interleukin-13

109 a.a.
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  • ￥1080 - 79695
  • Prospecbio
  • 以色列
  • cyt-182
  • 2025年12月25日
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    上海经科化学科技有限公司
    16 年钻石会员

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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件：

      for future use below -18°C

    • 保质期：

      See instructions

    • 英文名：

      rIL13 109 a.a.

    • 库存：

      部分小规格有备货

    • 供应商：

      上海经科化学科技有限公司

    • CAS号：

      无

    • 规格：

      2ug/10ug/1mg

    规格：2ug产品价格：￥1080.0
    规格：10ug产品价格：￥2415.0
    规格：1mg产品价格：￥79695.0

    产品细节图片1

    CATALOGUE NUMBER

    CYT-182

    SYNONYMS

    NC300, ALRH, BHR1, P600, IL-13.

    INTRODUCTION

    IL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. IL-13 is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.

    DESCRIPTION

    Interleukin-13 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 109 amino acids and having a molecular mass of 11.9 kDa.
    The IL-13 is purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    The protein (1mg/ml) was lyophilized in PBS, pH7.4.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized Interleukin 13 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    STABILITY

    Lyophilized Interleukin-13 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL13 should be stored at 4°C between 2-7 days and for future use below -18°C.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please prevent freeze-thaw cycles.

    PURITY

    Greater than 95% as determined by SDS-PAGE.

    AMINO ACID SEQUENCE

    VRRSTSPPVA LRELIEELSN ITQDQKTSLC NSSIVWSVDI TAGGFCAALE SLTNISSCNA IHRTQRILNG LCNQKASDVA SSPPDTKIEV AQFISKLLNY SKQLFRYGH.

    BIOLOGICAL ACTIVITY

    ED50 range = 40ng/ml, corresponding to a specific activity of > 25,000IU/mg as determined by the dose dependent proliferation of human TF-1 cells. Optimal concentration for individual application should be determined by a dose response assay.

    PROTEIN CONTENT

    Protein quantitation was carried out by two independent methods:
    1. UV spectroscopy at 280 nm using the absorbency value of 0.69 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
    2. Analysis by RP-HPLC, using a calibrated solution of IL-13 as a Reference Standard.

    SAFETY DATA SHEET

    SDS

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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    图标文献和实验
    该产品被引用文献

    Interleukin-13 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 109 amino acids and having a molecular mass of 11.9 kDa.
    The IL-13 is purified by proprietary chromatographic techniques.

    相关实验

    • Dual Vector Expansion of the Recombinant AAV Packaging Capacity

      vectors such as adenovirus and herpes simplex virus (13 ). Regulated myocardial transgene expression has also been demonstrated with an AAV vector containing glucocorticoid response elements in rat heart (14 ).

    • 【申请上传】Current Protocols in Immunology

      Unit 6.18 Measurement of Interleukin-13 Unit 6.19 ELISPOT Assay to Detect Cytokine-Secreting Murine and Human Cells Unit 6.20 Immunoenzymetric Assay of Mouse and Human Cytokines Using NIP-Labeled Anti-Cytokine Antibodies Unit 6.21 Detection

    • 帮忙看看我用ppxa0049设计的引物

      /antiapoptotic effects of IL-13 in liver transplantation. REFERENCE 32 (bases 1 to 1600) AUTHORS Jonas,J.C., Guiot,Y., Rahier,J. and Henquin,J.C. TITLE Haeme-oxygenase 1 expression in rat pancreatic beta cells is stimulated by supraphysiological glucose

    查看更多相关实验 >

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    文献支持
    Recombinant Rat Interleukin-13 109 a.a.
    ￥1080 - 79695