• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        【申请上传】Current Protocols in Immunology

        丁香园论坛

        8287
        Current Protocols in Immunology
        47.7 MB (50,069,504 字节)

        Current Protocols in Immunology
        Chapter 1 Care and Handling of Laboratory Animals
        Introduction
        Section I Animal Health and Husbandry
        Unit 1.1 Animal Health Assurance
        Unit 1.2 Managing Immunocompromised Animals
        Unit 1.3 Handling and Restraint
        Unit 1.4 Anesthesia
        Unit 1.5 Animal Identification
        Section II Use of Mouse, Rat, Hamster, and Rabbit
        Unit 1.6 Parenteral Injections
        Unit 1.7 Blood Collection
        Unit 1.8 Euthanasia
        Section III Selected Surgical Procedures
        Unit 1.9 Removal of Lymphoid Organs
        Unit 1.10 Survival Surgery: Removal of the Spleen or Thymus
        Unit 1.11 Surgical Removal of Mouse Salivary Glands
        Chapter 2 Induction of Immune Responses
        Introduction
        Section I Assays for Antibody Production
        Unit 2.1 Enzyme-Linked Immunosorbent Assays
        Unit 2.2 Isotype Determination of Antibodies
        Unit 2.3 Double-Immunodiffusion Assay for Detecting Specific Antibodies
        Section II Production of Antibodies
        Unit 2.4 Production of Polyclonal Antisera
        Unit 2.5 Production of Monoclonal Antibodies
        Unit 2.6 Monoclonal Antibody Supernatant and Ascites Fluid Production
        Section III Purification and Fragmentation of Antibodies
        Unit 2.7 Purification of Immunoglobulin G
        Unit 2.8 Fragmentation of Immunoglobulin G
        Unit 2.9 Purification of Immunoglobulin M and Immunoglobulin D
        Unit 2.10A Fragmentation of Immunoglobulin M
        Unit 2.10B Purification of Human IgA
        Section IV Specialized Techniques
        Unit 2.11 Preparation of Immune Stimulating Complexes (ISCOMs as Adjuvants
        Unit 2.12 Cloning, Expression, and Modification of Antibody V Regions
        Unit 2.13 Production of Bispecific Antibodies
        Unit 2.14 Nucleic Acid Immunizations
        Chapter 3 In Vitro Assays for Mouse Lymphocyte Function
        Introduction
        Section I Isolation and Fractionation of Mononuclear Cell Populations
        Unit 3.1 Isolation of Mouse Mononuclear Cells
        Unit 3.2 T Cell Enrichment By Nonadherence to Nylon
        Unit 3.3 T Cell Enrichment by Cytotoxic Elimination of B Cells and Accessory Cells
        Unit 3.4 T Cell Depletion by Cytotoxic Elimination
        Unit 3.5A Fractionation of T and B Cells Using Magnetic Beads
        Unit 3.5B Isolation of Ly-1+/CD5+ B Cells by Cell Sorting
        Unit 3.6 Depletion of Accessory Cells by Adherence to Sephadex G-10
        Unit 3.7 Isolation of Dendritic Cells
        Section II Assays for B Cell Function
        Unit 3.8 Assays for B Lymphocyte Function
        Unit 3.9 Early Events in B Lymphocyte Activation
        Unit 3.10 Proliferative Assays for B Cell Function
        Section III Assays for T Cell Function
        Unit 3.11 Induction and Measurement of Cytotoxic T Lymphocyte Activity
        Unit 3.12 Proliferative Assays for T Cell Function
        Unit 3.13 Production of T Cell Clones
        Unit 3.14 Production of Mouse T Cell Hybridomas
        Unit 3.15 Quantitation of Functional T Cells by Limiting Dilution
        Unit 3.16 Granule Enzyme Exocytosis Assay for Cytotoxic T Lymphocyte Activation
        Section IV Related Isolation Procedures and Functional Assays
        Unit 3.17 Measurement of Apoptosis and Other Forms of Cell Death
        Unit 3.18 Fetal Thymus Organ Culture for T Cell Development Studies
        Unit 3.19 Isolation of Mouse Small Intestinal Intraepithelial Lymphocytes, Peyer's Patch, and Lamina
        Propria Cells
        Unit 3.20 Isolation of Mouse Neutrophils
        Unit 3.21 Isolation of Mouse Intrahepatic Lymphocytes
        Unit 3.22 Isolation of Murine Natural Killer Cells
        Chapter 4 In Vivo Assays for Lymphocyte Function
        Introduction
        Unit 4.1 In Vivo Depletion of CD4- and CD8-Specific T Cells
        Unit 4.2 Contact Hypersensitivity
        Unit 4.3 Animal Models of Acute and Chronic Graft-Versus-Host Disease
        Unit 4.4 Skin Allograft Rejection1
        Unit 4.5 Delayed-Type Hypersensitivity
        Unit 4.6 Assessment of Lymphocyte Development in Radiation Bone Marrow Chimeras
        Unit 4.7 Measurement of T and B Cell Turnover with Bromodeoxyuridine
        Unit 4.8 Construction of Human-SCID Chimeric Mice
        Unit 4.9 Use of the Intracellular Fluorescent Dye CFSE to Monitor Lymphocyte Migration and Proliferation
        Chapter 5 Immunofluorescence and Cell Sorting
        Introduction
        Unit 5.1 Overview of Flow Cytometry
        Unit 5.2 Analysis of Flow Cytometry Data
        Unit 5.3 Preparation of Cells and Reagents for Flow Cytometry
        Unit 5.4 Flow Cytometry Analysis Using the Becton Dickinson FACS Calibur
        Unit 5.5 Measurement of Intracellular Ions by Flow Cytometry
        Unit 5.6 Measurement of Intercellular Conjugates by Flow Cytometry
        Unit 5.7 Analysis of Cellular DNA Content by Flow Cytometry
        Unit 5.8 Multiparameter FACS Analysis
        Chapter 6 Cytokines and Their Cellular Receptors
        Introduction
        Unit 6.1 Measurement of Lymphokine Receptors
        Unit 6.2 Measurement of Soluble and Membrane-Bound Interleukin 1 Using a Fibroblast Bioassay
        Unit 6.3 Measurement of Human and Murine Interleukin 2 and Interleukin 4
        Unit 6.4 Measurement of Interleukin 3 and Other Hematopoietic Cytokines, such as GM-CSF, G-CSF, M-CSF,
        Erythropoietin, Steel Factor, and Flt-3 Ligand
        Unit 6.5 Measurement of Mouse and Human Interleukin 5
        Unit 6.6 Measurement of Interleukin 6
        Unit 6.7 Measurement of Interleukin 7
        Unit 6.8 Measurement of Mouse and Human Interferon
        Unit 6.9 Measurement of Antiviral Activity Induced by Interferons , , and
        Unit 6.10 Measurement of Tumor Necrosis Factor and
        Unit 6.11 Measurement of TGF- in Biological Fluids
        Unit 6.12 Biological Responses to Chemokine Superfamily Members
        Unit 6.13 Measurement of Mouse and Human Interleukin 9
        Unit 6.14 Measurement of Mouse and Human Interleukin 10
        Unit 6.15 Measurement of Human Interleukin 11
        Unit 6.16 Measurement of Human and Mouse Interleukin-12
        Unit 6.17 Measurement of Human and Murine Stem Cell Factor (c-kit Ligand
        Unit 6.18 Measurement of Interleukin-13
        Unit 6.19 ELISPOT Assay to Detect Cytokine-Secreting Murine and Human Cells
        Unit 6.20 Immunoenzymetric Assay of Mouse and Human Cytokines Using NIP-Labeled Anti-Cytokine
        Antibodies
        Unit 6.21 Detection of Cytokine Receptors by Flow Cytometry
        Unit 6.22 Measurement of Interleukin 15
        Unit 6.23 Measurement of Interleukin 16
        Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry
        Unit 6.25 Measurement of Interleukin 17
        Unit 6.26 Measurement of Human and Mouse Interleukin 18
        Unit 6.27 Detection and Isolation of Cytokine Secreting Cells Using the Cytometric Cytokine Secretion
        Assay
        Unit 6.28 The In Vivo Cytokine Capture Assay for Measurement of Cytokine Production in the Mouse
        Unit 6.29 Cytokine, Growth Factor, and Chemokine Ligand Database
        Chapter 7 Immunologic Studies in Humans
        Introduction
        Section I Preparation of Human Mononuclear Cell Populations and Subpopulations
        Unit 7.1 Isolation of Whole Mononuclear Cells from Peripheral Blood and Cord Blood
        Unit 7.2 Isolation of T Cells Using Rosetting Procedures
        Unit 7.3 Purification of T Cell Subpopulations
        Unit 7.4 Immunomagnetic Purification of T Cell Subpopulations
        Unit 7.5 Isolation of B Cell Populations
        Unit 7.6 Isolation of Monocyte/Macrophage Populations
        Unit 7.7 Isolation of Human NK Cells and Generation of LAK Activity
        Unit 7.8 Isolation of Mononuclear Cells From Tonsillar Tissue
        Unit 7.9 Detection of Unseparated Human Lymphocytes by Flow Cytometry
        Section II Measurement of Basic Immunologic Characteristics of Human Mononuclear Cells
        Unit 7.10 Measurement of Proliferative Responses of Cultured Lymphocytes
        Unit 7.11 Induction of Polyclonal Immunoglobulin Synthesis
        Unit 7.12 Measurement of Polyclonal Immunoglobulin Synthesis Using ELISA
        Unit 7.13 Measurement of Polyclonal Immunoglobulin Synthesis Using the Reverse Plaque Technique
        Unit 7.14 Measurement of Immunoglobulin Synthesis Using the ELISPOT Assay
        Unit 7.15 Measurement of In Vitro Specific-Antibody Synthesis
        Unit 7.16 Induction and Measurement of In Vivo Antibody Responses
        Unit 7.17 Measurement of Polyclonal and Antigen-Specific Cytotoxic T Cell Function
        Unit 7.18 Measurement of Cytotoxic Activity of NK/LAK Cells
        Section III Long-Term and Immortalized Human Cell Lines and Clones
        Unit 7.19 Generation and Maintenance of Cloned Human T Cell Lines
        Unit 7.20 Generation of HTLV-I-Transformed T Cell Lines
        Unit 7.21 Generation of T Cell Hybridomas
        Unit 7.22 Generation of Epstein-Barr Virus (EBV—Immortalized B Cell Lines
        Section IV Preparation and Functional Analysis of Other Human Lymphoid And Nonlymphoid
        Cells
        Unit 7.23 Isolation and Functional Analysis of Neutrophils
        Unit 7.24 Isolation of Human Basophils
        Unit 7.25 Isolation of Tissue Mast Cells
        Unit 7.26 Measuring Degranulation of Mast Cells
        Unit 7.27 51Cr Release Assay of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC
        Unit 7.28 Measurement of Cellular Adhesion Under Static Conditions
        Unit 7.29 Cell Adhesion Under Hydrodynamic Flow Conditions
        Unit 7.30 Isolation of Human Intestinal Mucosal Mononuclear Cells
        Unit 7.31 Isolation of Human Eosinophils
        Unit 7.32 Isolation and Generation of Human Dendritic Cells
        Unit 7.33 Measurement of Prostaglandins and Other Eicosanoids
        Unit 7.34 Isolation and Characterization of Human Natural Killer Cell Subsets
        Chapter 8 Isolation and Analysis of Proteins
        Introduction
        Section I Solubilization of Cellular Proteins
        Unit 8.1A Solubilization of Lymphocytes
        Unit 8.1B Purification of Organelles from Mammalian Cells
        Unit 8.1C Subcellular Fractionation of Tissue Culture Cells
        Section II Isolation of Proteins Using Antibodies
        Unit 8.2 Immunoaffinity Chromatography
        Unit 8.3 Immunoprecipitation
        Section III Electrophoretic Separation of Proteins
        Unit 8.4 One-Dimensional Gel Electrophoresis of Proteins
        Unit 8.5 Two-Dimensional Gel Electrophoresis Using the O'Farrell System
        Unit 8.6 Diagonal Gel Electrophoresis
        Unit 8.7 Isolation of Proteins for Microsequence Analysis
        Unit 8.8 Electroelution of Proteins from Stained Gels
        Section IV Detection of Proteins
        Unit 8.9 Staining Proteins in Gels
        Unit 8.10 Immunoblotting and Immunodetection
        Unit 8.11 Iodination of Soluble and Membrane-Bound Proteins
        Unit 8.12 Biosynthetic Labeling of Proteins
        Unit 8.13 Metabolic Radiolabeling of Animal Cell Glycoconjugates
        Unit 8.14 Inhibition of N-Linked Glycosylation
        Unit 8.15 Release of Saccharides from Glycoconjugates
        Unit 8.16 Biotinylation and Analysis of Membrane-Bound and Soluble Proteins
        Chapter 9 Peptides
        Introduction
        Unit 9.1 Introduction to Peptide Synthesis
        Unit 9.2 Synthetic Peptides for Production of Antibodies that Recognize Intact Proteins
        Unit 9.3 Production of Anti-Peptide Antisera
        Unit 9.4 B Cell Epitope Mapping Using Synthetic Peptides
        Unit 9.5 Identification of B Cell and T Cell Epitopes Using Synthetic Peptide Combinatorial Libraries
        Unit 9.6 Synthesis and Application of Peptide Dendrimers As Protein Mimetics
        Unit 9.7 Synthesis of Multiple Peptides on Plastic Pins
        Unit 9.8 Phage Display Selection and Analysis of Ab-Binding Epitopes
        Chapter 10 Molecular Biology
        Introduction
        Section I Isolation and Purification of DNA
        Unit 10.1 Purification and Concentration of DNA from Aqueous Solutions
        Unit 10.2 Preparation of Genomic DNA from Mammalian Tissue
        Unit 10.3 Preparation of Bacterial Plasmid DNA
        Section II Resolution and Recovery of DNA Fragments
        Unit 10.4 Agarose Gel Electrophoresis
        Unit 10.5 Isolation and Purification of Large DNA Restriction Fragments from Agarose Gels
        Section III Analysis of Genomic DNA by Blotting and Hybridization
        Unit 10.6A Southern Blotting
        Unit 10.6B Hybridization Analysis of DNA Blots
        Unit 10.7 Deprotection of Oligonucleotides and Purification Using Denaturing PAGE
        Section IV Enzymatic Cleavage and Labeling of DNA
        Unit 10.8 Digestion of DNA with Restriction Endonucleases
        Unit 10.9 Reagents and Radioisotopes Used to Manipulate Nucleic Acids
        Unit 10.10 Enzymatic Labeling of Nucleic Acids
        Section V Preparation and Analysis of RNA
        Unit 10.11 Preparation of RNA from Tissues and Cells
        Unit 10.12 Analysis of RNA by Northern and Slot-Blot Hybridization
        Section VI Transfection and Expression of Cloned DNA
        Unit 10.13 Calcium Phosphate Transfection
        Unit 10.14 Transfection Using DEAE-Dextran
        Unit 10.15 Transfection by Electroporation
        Unit 10.16 Liposome-Mediated Transfection
        Unit 10.17A Stable Transfer of Genes into Mammalian Cells
        Unit 10.17B Transfection of Lymphoid Cells
        Unit 10.17C Rapid Production of Retroviruses for Efficient Gene Delivery to Mammalian Cells Using 293T
        Cell—Based Systems
        Unit 10.18 Use of Monoclonal Antibodies for Expression Cloning
        Unit 10.19A Construction of Immunoglobulin Fusion Proteins
        Unit 10.19B Phage Display of Single-Chain Antibody Constructs
        Section VII The Polymerase Chain Reaction
        Unit 10.20 Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
        Unit 10.21 Quantitation of Rare DNAs by PCR
        Unit 10.22 Detection of Isotype Switch Rearrangement in Bulk Culture by PCR
        Unit 10.23 Detection of Cytokine mRNA Expression by PCR
        Unit 10.24 cDNA Amplification Using One-Sided (Anchored PCR
        Unit 10.25 Double-Stranded DNA Sequence Analysis
        Unit 10.26 Evaluating Human T Cell Receptor Gene Expression by PCR
        Unit 10.27 Molecular Analysis of Mouse T Cell Receptor Expression Using PCR
        Unit 10.28 Spectratype/Immunoscope Analysis of the Expressed TCR Repertoire
        Unit 10.29 Multiprobe Ribonuclease Protection Assay for Simultaneous Measurement of mRNA Expression
        Unit 10.30 Analysis of Telomere Length and Telomerase Activity
        Chapter 11 Biochemistry of Cell Activation
        Introduction
        Unit 11.1 Analysis of Inositol Phospholipid Turnover During Lymphocyte Activation
        Unit 11.2 Preparation and Analysis of Phosphorylated Proteins
        Unit 11.3 Antiphosphotyrosine Blotting
        Unit 11.4 Immune-Complex Assays for Tyrosine Protein Kinases
        Unit 11.5 Assessment of the Effects of Tyrosine Protein Kinase Inhibitors
        Unit 11.6 Production of Antibodies that Recognize Specific Tyrosine-Phosphorylated Peptides
        Unit 11.7 Using Protein Kinase and Protein Phosphatase Inhibitors to Dissect Protein Phosphorylation
        Pathways
        Unit 11.8 Analyzing Mitogen-Activated Protein Kinase (MAPK Activities in T Cells
        Unit 11.9A Key Molecules Involved in Receptor-Mediated Lymphocyte Activation
        Unit 11.9B Signal Transduction During Natural Killer Cell Activation
        Unit 11.9C Apoptosis Signaling Pathways
        Unit 11.9D TRAF-Mediated TNFR-Family Signaling
        Unit 11.10 Isolation and Use of Rafts
        Chapter 12 Detection and Analysis of HIV
        Introduction
        Unit 12.1 General Guidelines for Experimenting with HIV
        Unit 12.2 Isolation and Quantitation of HIV in Peripheral Blood
        Unit 12.3 Infection of CD4+ Primary Cell Lines and Induction of HIV
        Unit 12.4 Culture of HIV in Monocytes and Macrophages
        Unit 12.5 Detection Assays for HIV Proteins
        Unit 12.6 Detection of HIV DNA and RNA Using PCR
        Unit 12.7 Regulation of the HIV Promoter/Enhancer
        Unit 12.8 In Situ Hybridization for Detection of HIV RNA
        Unit 12.9 In Vitro Evaluation of Experimental Agents for Anti-HIV Activity
        Unit 12.10 Vaccinia-Based Reporter Gene Cell-Fusion Assays to Quantitate Functional Interactions of HIV
        Envelope Glycoprotein with Receptors
        Chapter 13 Complement
        Introduction
        Unit 13.1 Classical Pathway Evaluation
        Unit 13.2 Alternative Pathway Evaluation
        Unit 13.3 Isolation and Purification of C3 from Human Plasma
        Unit 13.4 Assays for Membrane Complement Receptors
        Unit 13.5 Isolation and Functional Assay of the Membrane Complement Inhibitors CD55 (DAF and CD59
        (MIRL
        Unit 13.6 Assays for the Mannan-Binding Lectin Pathway
        Chapter 14 Innate Immunity
        Introduction
        Unit 14.1 Isolation of Murine Macrophages
        Unit 14.2 Characterization of Murine Macrophages
        Unit 14.3 Characterization of Human Monocytes/Macrophages
        Unit 14.4 Activation of Murine Macrophages
        Unit 14.5 Oxidative Metabolism of Murine Macrophages
        Unit 14.6 Measurement of Bacterial Ingestion and Killing by Macrophages
        Unit 14.7 Assay for Macrophage-Mediated Anti-Tumor Cytotoxicity
        Unit 14.8 Measurement of Fc Receptor—Mediated Binding and Phagocytosis
        Unit 14.9 Measurement of Interferon-Mediated Antiviral Activity of Macrophages
        Unit 14.10 Human Natural Killer Receptors and Their Ligands
        Unit 14.11 Isolation and Functional Use of Human NK T Cells
        Unit 14.12 Toll Receptors
        Chapter 15 Animal Models for Autoimmune and Inflammatory Disease
        Introduction
        Unit 15.1 Experimental Autoimmune Encephalomyelitis in the Mouse
        Unit 15.2 Experimental Autoimmune Encephalomyelitis in the Rat
        Unit 15.3 The BB Rat as a Model of Human Insulin-Dependent Diabetes Mellitus
        Unit 15.4 Adjuvant Arthritis in the Rat
        Unit 15.5 Collagen-Induced Arthritis
        Unit 15.6 Experimental Autoimmune Uveoretinitis in the Rat and Mouse
        Unit 15.7 Experimental Autoimmune Thyroiditis in the Mouse
        Unit 15.8 Experimental Autoimmune Myasthenia Gravis in the Mouse
        Unit 15.9 The NOD Mouse: A Model for Insulin-Dependent Diabetes Mellitus
        Unit 15.10 Streptococcal Cell Wall Arthritis
        Unit 15.11 Animal Models of IgA Nephropathy
        Unit 15.12 Induction of Autoimmune Disease by Depletion of Regulatory T Cells
        Unit 15.13 Induction of Inflammatory Bowel Disease in Immunodeficient Mice by Depletion of Regulatory T
        Cells
        Unit 15.14 Autoimmune Myocarditis
        Unit 15.15 Mercuric Chloride—Induced Autoimmunity
        Unit 15.16 The Day-3 Thymectomy Model for Induction of Multiple Organ-Specific Autoimmune Diseases
        Unit 15.17 Autoimmune Ovarian Disease Induced by Immunization with Zona Pellucida (ZP3 Peptide
        Unit 15.18 Animal Models of Airway Sensitization
        Unit 15.19 Induction of TNBS Colitis in Mice
        Unit 15.20 Animal Models for SLE
        Chapter 16 Antigen Processing and Presentation
        Introduction
        Unit 16.1 Choosing and Preparing Antigen-Presenting Cells
        Unit 16.2 Presenting Exogenous Antigen to T Cells
        Chapter 17 Engineering Immune Molecules and Receptors
        Introduction
        Unit 17.1 Bacteriophage Library Construction and Selection of Recombinant Antibodies
        Unit 17.2 Monitoring Antigen-Specific T Cells Using MHC-Ig Dimers
        Unit 17.3 MHC-Peptide Tetramers to Visualize Antigen-Specific T Cells
        Chapter 18 Ligand-Receptor Interactions in the Immune System
        Introduction
        Unit 18.1 Binding of Biotinylated Peptides to MHC Class II Proteins on Cell Surfaces
        Unit 18.2 Detection of Low-Affinity Ligand-Receptor Interactions at the Cell Surface with Fluorescent
        Microspheres
        Unit 18.3 Measurement of MHC/Peptide Interactions by Gel Filtration
        Unit 18.4 Measurement of Peptide-MHC Interactions in Solution Using the Spin Column Filtration Assay
        Unit 18.5 Measurement of the Kinetics of Biomolecular Interactions Using the IAsys Resonant Mirror
        Biosensor
        Unit 18.6 Affinity-Based Biosensors for Biomolecular Interaction Analysis
        Unit 18.7 Biotin Labeling and Quantitation of Cell-Surface Proteins
        Unit 18.8 Measuring Protein-Protein Interactions by Equilibrium Sedimentation
        Unit 18.9 Binding of Small Peptides to Immobilized Antibodies: Kinetic Analysis by Surface Plasmon
        Resonance
        Unit 18.10 Fluorescence Resonance Energy Transfer: Techniques for Measuring Molecular Conformation
        and Molecular Proximity
        Unit 18.11 Peptide Induction of Surface Expression of Class I MHC
        Unit 18.12 Competition-Based Cellular Peptide Binding Assay for HLA Class I
        Chapter 19 Animal Models for Infectious Diseases
        Introduction
        Unit 19.1 Schistosomiasis
        Unit 19.2 Animal Models for the Analysis of Immune Responses to Leishmaniasis
        Unit 19.3 Animal Models for Toxoplasma gondii Infection
        Unit 19.4 Experimental Asexual Blood Stage Malaria Immunity
        Unit 19.5 Animal Models of Mycobacteria Infection
        Unit 19.6 Animal Models for Candidiasis
        Unit 19.7 A Mouse Model for Cytomegalovirus Infection
        Unit 19.8 Helicobacter Animal Models
        Unit 19.9 Animal Model for Infection with Listeria monocytogenes
        Unit 19.10 Animal Models Using Lymphocytic Choriomeningitis Virus
        Unit 19.11 Influenza Virus
        Unit 19.12 Animal Model of Nippostrongylus brasiliensis and Heligmosomoides polygyrus
        Chapter 20 Animals Models for Tumor Immunology
        Introduction
        Unit 20.1 B16 as a Mouse Model for Human Melanoma
        Unit 20.2 Mouse 4T1 Breast Tumor Model
        Unit 20.3 Mouse SaI Sarcoma Tumor Model
        Unit 20.4 The P815 Mastocytoma Tumor Model
        Unit 20.5 The TRAMP Mouse as a Model for Prostate Cancer1
        Unit 20.6 Models for Lymphoma
        Chapter 21 Microscopy
        Introduction
        Unit 21.1 Proper Alignment and Adjustment of the Light Microscope
        Unit 21.2 Fluorescence Microscopy
        Unit 21.3 Immunofluorescence Staining
        Unit 21.4 Immunohistochemistry
        Appendix 1 Abbreviations and Useful Data
        1A Abbreviations Used in this Manual
        1B Amino Acids
        1C Commonly Used Mouse Strains
        1D Commonly Used Detergents
        1E Murine Models of Immunodeficiency and Autoimmune Disease
        1F Mouse Endogenous Superantigens: Ms and Mls-like Determinants Encoded by Mouse Retroviruses
        1G HLA Type of EBV-Transformed Human B Cell Lines
        1H Commonly Used Rat Strains
        1I Peptide Binding Motifs for MHC Class I and II Molecules
        1J Web Sites of Interest to Immunologists
        1K Human MHC Genes and Products
        1L Mouse MHC Genes and Products
        1M Listing, Location, Binding Motifs, and Expression of Nonclassical Class I and Related Genes and
        Molecules
        1N Overview of Protein Folds in the Immune System1
        1O Nomenclature of the Human T Cell Receptor Genes
        1P Nomenclature of the Human Immunoglobulin Genes
        1Q Safe Use of Radioisotopes
        1R Receptors Involved in the Regulation of Murine NK Cells
        1S Nomenclature and Serology of HLA Class I and Class II Alleles
        1T Heat-Shock Proteins
        1U Large-Scale Cell Culture
        Appendix 2 Commonly Used Reagents and Equipment
        2A Common Media, Buffers, and Stock Solutions
        2B EquipmentAppendix 3 Commonly Used Immunological Techniques
        3A Monitoring Cell Growth
        3B Trypan Blue Exclusion Test of Cell Viability
        3C Wright-Giemsa and Nonspecific Esterase Staining of Cells
        3D Labeling Cells in Microtiter Plates for Determination of [3H]Thymidine Uptake
        3E Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures
        3F Obtaining Human Peripheral Blood Cells
        3G Cryopreservation of Cells
        3H Dialysis and Concentration of Protein Solutions
        3I Column Chromatography of Antibodies and Fragments
        3J Detection and Quantitation of Radiolabeled Proteins and DNA in Gels and Blots
        3K Silanizing Glassware
        3L Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
        3M Preparing Frozen Bacterial Stocks
        3N Transformation of E. coli by Electroporation
        3O Gram Staining
        Appendix 4 The CD System of Leukocyte Surface Molecules
        4A Monoclonal Antibodies to Human Cell Surface Antigens
        4B Monoclonal Antibodies to Mouse Cell-Surface Antigens
        4C Monoclonal Antibodies to Rat Leukocyte Surface Antigens, MHC Antigens, and Immunoglobulins
        Appendix 5
        Selected Suppliers of Reagents and Equipment
        Jingler 20041020
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序