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- 技术资料
- 亚型:
Rat IgG2b, κ
- 形态:
液体
- 保存条件:
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 克隆性:
Monoclonal
- 标记物:
PE
- 适应物种:
Mouse
- 保质期:
12个月
- 目录编号:
E-AB-F1013D
- 级别:
科研
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 宿主:
Rat
- 应用范围:
FCM
- 抗体英文名:
PE Anti-Mouse CD3 Antibody[17A2]_货号:E-AB-F1013D
- 抗体名:
PE Anti-Mouse CD3 Antibody流式抗体[17A2]_货号:E-AB-F1013D
- 规格:
100Tests/50Tests/100Tests×2
| 规格: | 100Tests | 产品价格: | ¥396.0 |
|---|---|---|---|
| 规格: | 50Tests | 产品价格: | ¥240.0 |
| 规格: | 100Tests×2 | 产品价格: | ¥684.0 |
产品名称:PE Anti-Mouse CD3 Antibody[17A2]
货号:E-AB-F1013D
| Background |
CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
|
| Alternate Names |
T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain;CD3E/D/G/Z;CD3e/d/g/z;CD3E/D/G/Z;CD3;
|
| Swissprot |
P04235;P11942;P22646;P24161;
|
| Gene ID |
12502
|
| Clone No |
17A2
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2b, κ
|
| Isotype Control |
PE Rat IgG2b, κ Isotype Control[LTF-2]
|
| Conjugation |
PE
|
| Conjugation Information |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验-
Self-Propelled Bacteria-Mediated In Situ Biosynthesis of MnS Nanoparticles for Remodeling Tumor Microenvironment to Amplify cGAS-STING Pathway for Antitumor Immunity
IF: 18.5Journal: ADVANCED FUNCTIONAL MATERIALS (2025)DOI:10.1002/adfm.202503733Reactivity:Mouse
- 3) 及 CD45 -PE (HI30,白细胞标志) 抗体染色后,进行流式分析,死细胞以 DAPI 染色先行排除,左右分别为分选前后的细胞群分布。 总结: 几次实验的结果,使用 Fab-TACS® Gravity column 来从全血直接进行 CD3+ T 细胞分选,平均得率为 84.5%,纯度大于 90%,细胞存活率高于 95%,且目标细胞不含标记,贴近天然,使下游实验更精确。
7211011 小鼠淋巴细胞分离液 达优 100339 Ultra-LEAF™ Purified anti-mouse CD3ε Antibody BioLegend 102115 Ultra-LEAF™ Purified anti-mouse CD28 Antibody BioLegend CD3/CD28 Streptamer® 多聚体法: 货号 名称
: 由此步骤开始,流出的溶液包含目标细胞,请使用新的试剂管收集!首先,加入 1 ml 的 biotin 洗脱液,孵育 2 min。其次,加入 10 ml biotin 洗脱液,洗脱目标细胞。d) 再加入 10 ml biotin 洗脱液,洗下剩余的目标细胞。最后两步骤所收集的液体含分选出的目标细胞。(4) 结果分析以下流式图为从 7.5 ml 全血中,选出 CD3+ T 细胞的数据。选后细胞以 CD3-APC(OKT-3) 及 CD45-PE(HI30,白细胞标志) 抗体染色后,进行流式分析,死细胞
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