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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Mouse spleen cells
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
- 克隆性:
Monoclonal
- 标记物:
PE
- 适应物种:
Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Mouse
- 目录编号:
GTX80265
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rat
- 应用范围:
FACS
- 浓度:
0.2 mg/ml
- 靶点:
CD8 alpha
- 抗体英文名:
CD8 alpha antibody [53-6.7] (PE)
- 抗体名:
CD8 alpha 抗体 [53-6.7] (PE)
- 规格:
100 μg
FACS analysis of C57Bl/6 splenocytes using GTX80265 CD8 alpha antibody [53-6.7] (PE).
Solid line : Primary antibody
Dashed line : PE rat IgG2a isotype control
Antibody amount : 0.5 μg
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文献和实验Nagashima T et al., Am J Pathol 2011 (PMID:21224059)
CD3/ CD4 /CD8 T cell subset counts
Description Procedure for CD3/ CD4 /CD8 T cell subset counts using CD3-FITC / CD4-PE / CD8-PECy5 antibody Procedure 1. One 5 ml round bottom tube (Falcon) is taken per blood sample to be analyzed. The tube is labeled, which includes
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Phycoerythrin conjugation protocol
Centricon 30 and Centriprep 30 centrifugal concentrators (Amicon) * Room temperature is about 22 °C Method 1. Preparation of PE 2. SPDP modification of PE 3. SMCC modification of antibody 4. DTT
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