• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        CD3/ CD4 /CD8 T cell subset counts

        互联网

        2538

        Description
        Procedure for CD3/ CD4 /CD8 T cell subset counts using CD3-FITC / CD4-PE / CD8-PECy5 antibody

        Procedure
        1. One 5 ml round bottom tube (Falcon) is taken per blood sample to be analyzed. The tube is labeled, which includes the sample ID, test to be performed and date.
        2. 15 ƒY´l of the Test antibody (CD3-FITC / CD8-PE / CD4-PECy5) (Cytognos) is added to each sample tube.
        3. 100 ƒY´l of blood sample is added to corresponding test tube by REVERSE PIPETTING and the tubes are capped or covered with Parafilm. The tubes are mixed gently by tapping and incubated in dark for 10 min at room temperature. The tubes are shaken intermittently during incubation.
        4. 2 ml of RBC lysing solution (1 x Quicklysis solution by Cytognos) is added to each tube. The tubes are then covered with Parafilm, mixed thoroughly and incubated in dark for 10 min at room temperature.
        5. Sample tubes are then acquired on the Flowcytometer.
        6. The tubes are analyzed immediately.
        7. If the samples are not going to be analyzed immediately, steps 1-4 are performed and the samples are stored at 40C in dark until further analysis. Step 5 is performed just before analysis.


        Recipes
        1. Round bottom tube (capacity 5 ml)
        2. Test antibody (CD3-FITC/ CD8-PE/ CD4-PECy5; Cytognos, Spain)
        3. Lysing solution (Quicklysis)

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序