pTRV1植物干扰质粒

基本信息

启动子:	CaMV 35S Promoter
复制子:	ori
质粒分类:	植物系列，RNAi载体
质粒大小:	6791bp
原核抗性:	Kan
克隆菌株:	DH5α
培养条件:	37℃，有氧 LB
表达宿主:	植物细胞
5'测序引物:	根据序列设计引物
3'测序引物:	根据序列设计引物

质粒简介

   pTRV1 Tobacco rattle virus (TRV) based cDNA clone was placed between the duplicated CaMV 35S promoter and the nopaline synthase terminator in a T-DNA vector

质粒图谱

 

质粒序列

以下是TRV1的序列，非全长。
 
        1 ataaaacatt tcaatccttt gaacgcggta gaacgtgcta attggatttt ggtgagaacg
       61 cggtagaacg tacttatcac ctacagtttt attttgtttt tctttttggt ttaatctatc
      121 cagcttagta ccgagtgggg gaaagtgact ggtgtgccta aaaccttttc tttgatactt
      181 tgtaaaaata catacagata caatggcgaa cggtaacttc aagttgtctc aattgctcaa
      241 tgtggacgag atgtctgctg agcagaggag tcatttcttt gacttgatgc tgactaaacc
      301 tgattgtgag atcgggcaaa tgatgcaaag agttgttgtt gataaagtcg atgacatgat
      361 tagagaaaga aagactaaag atccagtgat tgttcatgaa gttctttctc agaaggaaca
      421 gaacaagttg atggaaattt atcctgaatt caatatcgtg tttaaagacg acaaaaacat
      481 ggttcatggg tttgcggctg ctgagcgaaa actacaagct ttattgcttt tagatagagt
      541 tcctgctctg caagaggtgg atgacatcgg tggtcaatgg tcgttttggg taactagagg
      601 tgagaaaagg attcattcct gttgtccaaa tctagatatt cgggatgatc agagagaaat
      661 ttctcgacag atatttctta ctgctattgg tgatcaagct agaagtggta agagacagat
      721 gtcggagaat gagctgtgga tgtatgacca atttcgtgaa aatattgctg cgcctaacgc
      781 ggttaggtgc aataatacat atcagggttg tacatgtagg ggtttttctg atggtaagaa
      841 gaaaggcgcg cagtatgcga tagctcttca cagcctgtat gacttcaagt tgaaagactt
      901 gatggctact atggttgaga agaaaactaa agtggttcat gctgctatgc tttttgctcc
      961 tgaaagtatg ttagtggacg aaggtccatt accttctgtt gacggttact acatgaagaa
     1021 gaacgggaag atctatttcg gttttgagaa agatccttcc ttttcttaca ttcatgactg
     1081 ggaagagtac aagaagtatc tactggggaa gccagtgagt taccaaggga atgtgttcta
     1141 cttcgaaccg tggcaggtga gaggagacac aatgcttttt tcgatctaca ggatagctgg
     1201 agttccgagg aggtctctat catcgcaaga gtactaccga agaatatata tcagtagatg
     1261 ggaaaacatg gttgttgtcc caattttcga tctggtcgaa tcaacgcgag agttggtcaa
     1321 gaaagacctg tttgtagaga aacaattcat ggacaagtgt ttggattaca tagctaggtt
     1381 atctgaccag cagctgacca taagcaatgt taaatcatac ttgagttcaa ataattgggt
     1441 cttattcata aacggggcgg ccgtgaagaa caagcaaagt gtagattctc gagatttaca
     1501 gttgttggct caaactttgc tagtgaagga acaagtggcg agacctgtca tgagggagtt
     1561 gcgtgaagca attctgactg agacgaaacc tatcacgtca ttgactgatg tgctgggttt
     1621 aatatcaaga aaactgtgga agcagtttgc taacaagatc gcagtcggcg gattcgttgg
     1681 catggttggt actctaattg gattctatcc aaagaaggta ctaacctggg cgaaggacac
     1741 accaaatggt ccagaactat gttacgagaa ctcgcacaaa accaaggtga tagtatttct
     1801 gagtgttgtg tatgccattg gaggaatcac gcttatgcgt cgagacatcc gagatggact
     1861 ggtgaaaaaa ctatgtgata tgtttgatat caaacggggg gcccatgtct tagacgttga
     1921 gaatccgtgc cgctattatg aaatcaacga tttctttagc agtctgtatt cggcatctga
     1981 gtccggtgag accgttttac cagatttatc cgaggtaaaa gccaagtctg ataagctatt
     2041 gcagcagaag aaagaaatcg ctgacgagtt tctaagtgca aaattctcta actattctgg
     2101 cagttcggtg agaacttctc caccatcggt ggtcggttca tctcgaagcg gactgggtct
     2161 gttgttggaa gacagtaacg tgctgaccca agctagagtt ggagtttcaa gaaaggtaga
     2221 cgatgaggag atcatggagc agtttctgag tggtcttatt gacactgaag cagaaattga
     2281 cgaggttgtt ccagcctttt cagctgaatg tgaaagaggg gaaacaagcg gtacaaaggt
     2341 gttgtgtaaa cctttaacgc caccaggatt tgagaacgtg ttgccagctg tcaaaccttt
     2401 ggtcagcaaa ggaaaaacgg tcaaacgtgt cgattacttc caagtgatgg gaggtgagag
     2461 attaccaaaa aggccggttg tcagtggaga cgattctgtg gacgctagaa gagagtttct
     2521 gtactactta gatgcggaga gagtcgctca aaatgatgaa attatgtctc tgtatcgtga
     2581 ctattcgaga ggagttattc gaactggagg tcagaattac ccgcacggac tgggagtgtg
     2641 ggatgtggag atgaagaact ggtgcatacg tccagtggtc actgaacatg cttatgtgtt
     2701 ccaaccagac aaacgtatgg atgattggtc gggatactta gaagtggctg tttgggaacg
     2761 aggtatgttg gtcaacgact tcgcggtcga aaggatgagt gattatgtca tagtttgcga
     2821 tcagacgtat ctttgcaata acaggttgat cttggacaat ttaagtgccc tggatctagg
     2881 accagttaac tgttcttttg aattagttga cggtgtacct ggttgtggta agtcgacaat
     2941 gattgtcaac tcagctaatc cttgtgtcga tgtggttctc tctactggga gagcagcaac
     3001 cgacgacttg atcgagagat tcgcgagcaa aggttttcca tgcaaattga aaaggagagt
     3061 gaagacggtt gattcttttt tgatgcattg tgttgatggt tctttaaccg gagacgtgtt
     3121 gcatttcgat gaagctctca tggcccatgc tggtatggtg tacttttgcg ctcagatagc
     3181 tggtgctaaa cgatgtatct gtcaaggaga tcagaatcaa atttctttca agcctagggt
     3241 atctcaagtt gatttgaggt tttctagtct ggtcggaaag tttgacattg ttacagaaaa
     3301 aagagaaact tacagaagtc cagcagatgt ggctgccgta ttgaacaagt actatactgg
     3361 agatgtcaga acacataacg cgactgctaa ttcgatgacg gtgaggaaga ttgtgtctaa
     3421 agaacaggtt tctttgaagc ctggtgctca gtacataact ttccttcagt ctgagaagaa
     3481 ggagttggta aatttgttgg cattgaggaa agtggcagct aaagtgagta cagtacacga
     3541 gtcgcaagga gagacattca aagatgtagt cctagtcagg acgaaaccta cggatgactc
     3601 aatcgctaga ggtcgggagt acttaatcgt ggcgttgtcg cgtcacacac aatcacttgt
     3661 gtatgaaact gtgaaagagg acgatgtaag caaagagatc agggaaagtg ccgcgcttac
     3721 gaaggcggct ttggcaagat tttttgttac tgagaccgtc ttatgacggt ttcggtctag
     3781 gtttgatgtc tttagacatc atgaagggcc ttgcgccgtt ccagattcag gtacgattac
     3841 ggacttggag atgtggtacg acgctttgtt tccgggaaat tcgttaagag actcaagcct
     3901 agacgggtat ttggtggcaa cgactgattg caatttgcga ttagacaatg ttacgatcaa
     3961 aagtggaaac tggaaagaca agtttgctga aaaagaaacg tttctgaaac cggttattcg
     4021 tactgctatg cctgacaaaa ggaagactac tcagttggag agtttgttag cattgcagaa
     4081 aaggaaccaa gcggcacccg atctacaaga aaatgtgcac gcaacagttc taatcgaaga
     4141 gacgatgaag aagttgaaat ctgttgtcta cgatgtggga aaaattcggg ctgatcctat
     4201 tgtcaataga gctcaaatgg agagatggtg gagaaatcaa agcacagcgg tacaggctaa
     4261 ggtagtagca gatgtgagag agttacatga aatagactat tcgtcttaca tgtatatgat
     4321 caaatctgac gtgaaaccta agactgattt aacaccgcaa tttgaatact cagctctaca
     4381 gactgttgtg tatcacgaga agttgatcaa ctcgttgttc ggtccaattt tcaaagaaat
     4441 taatgaacgc aagttggatg ctatgcaacc acattttgtg ttcaacacga gaatgacatc
     4501 gagtgattta aacgatcgag tgaagttctt aaatacggaa gcggcttacg actttgttga
     4561 gatagacatg tctaaattcg acaagtcggc aaatcgcttc catttacaac tgcagctgga
     4621 gatttacagg ttatttgggc tagatgagtg ggcggccttc ctttgggagg tgtcgcacac
     4681 tcaaactact gtgagagata ttcaaaatgg tatgatggcg catatttggt accaacaaaa
     4741 gagtggagat gctgatactt ataatgcaaa ttcagataga acactgtgtg cactcttgtc
     4801 tgaattacca ttggagaaag cagtcatggt tacatatgga ggagatgact cactgattgc
     4861 gtttcctaga ggaacgcagt ttgttgatcc gtgtccaaag ttggctacta agtggaattt
     4921 cgagtgcaag atttttaagt acgatgtccc aatgttttgt gggaagttct tgcttaagac
     4981 gtcatcgtgt tacgagttcg tgccagatcc ggtaaaagtt ctgacgaagt tggggaaaaa
     5041 gagtataaag gatgtgcaac atttagccga gatctacatc tcgctgaatg attccaatag
     5101 agctcttggg aactacatgg tggtatccaa actgtccgag tctgtttcag accggtattt
     5161 gtacaaaggt gattctgttc atgcgctttg tgcgctatgg aagcatatta agagttttac
     5221 agctctgtgt acattattcc gagacgaaaa cgataaggaa ttgaacccgg ctaaggttga
     5281 ttggaagaag gcacagagag ctgtgtcaaa cttttacgac tggtaatatg gaagacaagt
     5341 cattggtcac cttgaagaag aagactttcg aagtctcaaa attctcaaat ctaggggcca
     5401 ttgaattgtt tgtggacggt aggaggaaga gaccgaagta ttttcacaga agaagagaaa
     5461 ctgtcctaaa tcatgttggt gggaagaaga gtgaacacaa gttagacgtt tttgaccaaa
     5521 gggattacaa aatgattaaa tcttacgcgt ttctaaagat agtaggtgta caactagttg
     5581 taacatcaca tctacctgca gatacgcctg ggttcattca aatcgatctg ttggattcga
     5641 gacttactga gaaaagaaag agaggaaaga ctattcagag attcaaagct cgagcttgcg
     5701 ataactgttc agttgcgcag tacaaggttg aatacagtat ttccacacag gagaacgtac
     5761 ttgatgtctg gaaggtgggt tgtatttctg agggcgttcc ggtctgtgac ggtacatacc
     5821 ctttcagtat cgaagtgtcg ctaatatggg ttgctactga ttcgactagg cgcctcaatg
     5881 tggaagaact gaacagttcg gattacattg aaggcgattt taccgatcaa gaggttttcg
     5941 gtgagttcat gtctttgaaa caagtggaga tgaagacgat tgaggcgaag tacgatggtc
     6001 cttacagacc agctactact agacctaagt cattattgtc aagtgaagat gttaagagag
     6061 cgtctaataa gaaaaactcg tcttaatgca taaagaaatt tattgtcaat atgacgtgtg
     6121 tactcaaggg ttgtgtgaat gaagtcactg ttcttggtca cgagacgtgt agtatcggtc
     6181 atgctaacaa attgcgaaag caagttgctg acatggttgg tgtcacacgt aggtgtgcgg
     6241 aaaataattg tggatggttt gtctgtgttg ttatcaatga ttttactttt gatgtgtata
     6301 attgttgtgg ccgtagtcac cttgaaaagt gtcgtaaacg tgttgaaaca agaaatcgag
     6361 aaatttggaa acaaattcga cgaaatcaag ctgaaaacat gtctgcgaca gctaaaaagt
     6421 ctcataattc gaagacctct aagaagaaat tcaaagagga cagagaattt gggacaccaa
     6481 aaagattttt aagagatgat gttcctttcg ggattgatcg tttgtttgct ttttgatttt
     6541 attttatatt gttatctgtt tctgtgtata gactgtttga gattggcgct tggccgactc
     6601 attgtcttac cataggggaa cggactttgt ttgtgttgtt attttatttg tattttatta
     6661 aaattctcaa tgatctgaaa aggcctcgag gctaagagat tattgggggg tgagtaagta
     6721 cttttaaagt gatgatggtt acaaaggcaa aaggggtaaa acccctcgcc tacgtaagcg
     6781 ttattacgcc c